Liu Ying, Xiong Tianyi, Ma Wenjie, He Xiulan, Jiang Yanan, Pan Cong, Yu Ping, Mao Lanqun
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, The Chinese Academy of Sciences (CAS), Beijing 100190, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
ACS Sens. 2025 Jan 24;10(1):480-487. doi: 10.1021/acssensors.4c02893. Epub 2025 Jan 6.
Iontronic sensors based on confined space have garnered significant attention due to their promising applications, ranging from single-cell analysis to studies. However, their limited sensitivity has constrained their effectiveness in studying molecular information during physiological and pathological processes. Here, we demonstrate an electrolyte-gated ionic transistor (EGIT) by integrating the confined ion transport behavior in a double-barreled micropipet with an electrolyte-gated transistor configuration, achieving highly sensitive and selective sensing. Our EGIT operates at a gate voltage of less than 1 V and can amplify ion current variations by up to 2 orders of magnitude. Both experimental methods and finite element simulations reveal that signal amplification stems from the intensified electric field. Thanks to the easily modified inner surface of the micropipet and the transistor configuration, we develop a highly sensitive and selective iontronic sensing platform for neurochemicals such as ATP, dopamine, and serotonin. More importantly, by utilizing this iontronic sensor, we successfully achieve the detection of trace ATP in rat striatum microdialysate. This study not only expands the scope of transistor technologies but also introduces a novel approach for constructing highly sensitive iontronic sensors, which hold potential applications in biochemical sensing, health monitoring, and disease diagnosis.
基于受限空间的离子电子传感器因其从单细胞分析到相关研究等一系列有前景的应用而备受关注。然而，其有限的灵敏度限制了它们在生理和病理过程中研究分子信息时的有效性。在此，我们通过将双管微吸管中的受限离子传输行为与电解质门控晶体管结构相结合，展示了一种电解质门控离子晶体管（EGIT），实现了高灵敏度和选择性传感。我们的EGIT在小于1 V的栅极电压下工作，能够将离子电流变化放大高达2个数量级。实验方法和有限元模拟均表明，信号放大源于增强的电场。得益于微吸管易于修饰的内表面和晶体管结构，我们开发了一种用于检测ATP、多巴胺和血清素等神经化学物质的高灵敏度和选择性离子电子传感平台。更重要的是，通过利用这种离子电子传感器，我们成功实现了对大鼠纹状体微透析液中痕量ATP的检测。这项研究不仅扩展了晶体管技术的范围，还引入了一种构建高灵敏度离子电子传感器的新方法，这些传感器在生化传感、健康监测和疾病诊断方面具有潜在应用。
