Univ. Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France; Department of Advanced Materials Science and Nanotechnology (AMSN), University of Science and Technology of Hanoi (USTH), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghĩa Đô, Cãu Giãy, Hanoi, Viet Nam.
Department of Inorganic Chemistry, School of Chemical Engineering, Hanoi University of Science and Technology (HUST), 1st Dai Co Viet Road, Hanoi, Viet Nam.
Biosens Bioelectron. 2019 Feb 15;127:118-125. doi: 10.1016/j.bios.2018.12.005. Epub 2018 Dec 15.
This work proposes an approach for Cu sensing in water which combines the selectivity of the Gly-Gly-His (GGH) peptide probe with the sensitivity of the electrolyte-gated organic field-effect transistor (EGOFET). The oligopeptide probe was immobilized onto the gate electrode of the transistor by electrooxidation of the primary amine of the glycine moiety. Cu complexation by the grafted GGH was at first electrochemically evidenced, using cyclic and square wave voltammetries, then it was demonstrated that GGH-functionalized EGOFETs can transduce Cu complexation through a significant threshold voltage shift and therefore a change in drain current. The limit of detection is ca. 10 M and the sensitivity in the linear range (10 - 10 M) is 1 mA dec (drain current variations).
这项工作提出了一种在水中检测 Cu 的方法，该方法结合了 Gly-Gly-His（GGH）肽探针的选择性和电解质门控有机场效应晶体管（EGOFET）的灵敏度。通过甘氨酸部分的伯胺的电化学氧化，将寡肽探针固定在晶体管的栅电极上。使用循环伏安法和方波伏安法首先电化学证明了接枝 GGH 与 Cu 的络合，然后证明 GGH 功能化的 EGOFET 可以通过显著的阈值电压偏移来转换 Cu 的络合，从而改变漏极电流。检测限约为 10 M，线性范围（10 - 10 M）内的灵敏度为 1 mA dec（漏极电流变化）。
