Cao Jia-Feng, Chen Jiong
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, School of Marine Sciences, Ningbo University, Ningbo, China.
Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo, China.
Front Immunol. 2024 Dec 23;15:1521080. doi: 10.3389/fimmu.2024.1521080. eCollection 2024.
Neutrophil extracellular traps (NETs) are crucial for the immune defense of many organisms, serving as a potent mechanism for neutrophils to capture and eliminate extracellular pathogens. While NETosis and its antimicrobial mechanisms have been well studied in mammals, research on NETs formation in teleost fish remains limited. In this study, we used the large yellow croaker () as the study model to investigate NETosis and its role in pathogen defense. Our results showed that infection with could induce NETosis. To further explore the underlying mechanism, we performed transcriptome analysis and western blotting, which revealed that triggers NETosis through activation of the autophagy pathway. Inhibition of autophagy significantly reduced NET production, highlighting its critical role in this process. Furthermore, our studies demonstrated that NETs exert a bacteriostatic effect, significantly suppressing the growth of . Taken together, our findings reveal that autophagy regulates NETosis in large yellow croaker and underscore the essential role of NETs in bacterial defense, providing new insights into immune responses in teleost fish.
中性粒细胞胞外陷阱（NETs）对许多生物体的免疫防御至关重要，是中性粒细胞捕获和清除细胞外病原体的有效机制。虽然NETosis及其抗菌机制在哺乳动物中已得到充分研究，但硬骨鱼中NETs形成的研究仍然有限。在本研究中，我们以大黄鱼为研究模型，研究NETosis及其在病原体防御中的作用。我们的结果表明，感染[病原体名称未给出]可诱导NETosis。为了进一步探究潜在机制，我们进行了转录组分析和蛋白质免疫印迹，结果显示[病原体名称未给出]通过激活自噬途径触发NETosis。抑制自噬显著降低了NET的产生，突出了其在这一过程中的关键作用。此外，我们的研究表明，NETs具有抑菌作用，能显著抑制[细菌名称未给出]的生长。综上所述，我们的研究结果揭示了自噬调节大黄鱼NETosis，并强调了NETs在细菌防御中的重要作用，为硬骨鱼的免疫反应提供了新的见解。
