Zheng Hui, Yang Yong, Wu Sizheng, Jia Fan, Jiang Jiani, Yu Lin, Ou Guangmei, Shu Man, Qin Wei
College of Horticulture, Xinjiang Agricultural University, Urumqi, China.
School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
Front Nutr. 2024 Dec 23;11:1495655. doi: 10.3389/fnut.2024.1495655. eCollection 2024.
The application of plant growth regulators is an effective method to enhance flavonoid content in certain fruits; however, there is limited research comparing the effects of different plant growth regulators. This study evaluated the impact of pre-harvest application with melatonin, 24-epibrassinolide, and methyl jasmonate on flavonoid content in blueberry fruit. All three plant growth regulators increased the total polyphenol content, total flavonoid content, antioxidant capacities, and the activities of key enzymes involved in flavonoid biosynthesis, including flavone synthase, flavanone 3-hydroxylase, flavonol synthase, anthocyanidin synthase, and leucoanthocyanidin reductase. Among these, melatonin exhibited the most significant effect. Further comparative analyses showed that 0.5 mM melatonin was particularly effective in promoting the accumulation of flavonols, anthocyanins, and flavanones, as well as specific compounds such as avicularin, astragalin, morin, and reynoutrin in blueberry fruit, whereas 1.0 μM 24-epibrassinolide was more effective in enhancing the accumulation of flavones and flavanols, such as quercetin, leucocyanidin, phloretin, and epicatechin. In general, melatonin resulted in a greater enhancement of flavonoid content in blueberry fruit compared to 24-epibrassinolide and methyl jasmonate. This study highlights the distinct effects of these three plant growth regulators on flavonoid accumulation in blueberry fruit, providing valuable insights for the production of high-quality blueberries.
施用植物生长调节剂是提高某些水果中黄酮类化合物含量的有效方法；然而，比较不同植物生长调节剂效果的研究有限。本研究评估了采前施用褪黑素、24-表油菜素内酯和茉莉酸甲酯对蓝莓果实中黄酮类化合物含量的影响。所有这三种植物生长调节剂均提高了总多酚含量、总黄酮含量、抗氧化能力以及黄酮类生物合成中关键酶的活性，这些酶包括黄酮合酶、黄烷酮3-羟化酶、黄酮醇合酶、花青素合酶和无色花青素还原酶。其中，褪黑素的效果最为显著。进一步的比较分析表明，0.5 mM褪黑素在促进蓝莓果实中黄酮醇、花青素和黄烷酮以及诸如扁蓄苷、紫云英苷、桑色素和鼠李素等特定化合物的积累方面特别有效，而1.0 μM 24-表油菜素内酯在增强黄酮和黄烷醇（如槲皮素、无色花青素、根皮素和表儿茶素）的积累方面更有效。总体而言，与24-表油菜素内酯和茉莉酸甲酯相比，褪黑素对蓝莓果实中黄酮类化合物含量的提高作用更大。本研究突出了这三种植物生长调节剂对蓝莓果实中黄酮类化合物积累的不同影响，为高品质蓝莓的生产提供了有价值的见解。
