Department of Horticulture, College of Plant Science, Jilin University, Changchun 130062, China.
Jilin Engineering Research Center for Crop Biotechnology Breeding, College of Plant Science, Jilin University, Changchun 130062, China.
Int J Mol Sci. 2024 Oct 17;25(20):11135. doi: 10.3390/ijms252011135.
Blueberries ( spp.) are extremely sensitive to drought stress. Flavonoids are crucial secondary metabolites that possess the ability to withstand drought stress. Therefore, improving the drought resistance of blueberries by increasing the flavonoid content is crucial for the development of the blueberry industry. To explore the underlying molecular mechanism of blueberry in adaptation to drought stress, we performed an integrated analysis of the metabolome and transcriptome of blueberry leaves under drought stress. We found that the most enriched drought-responsive genes are mainly involved in flavonoid biosynthesis and plant hormone signal transduction pathways based on transcriptome data and the main drought-responsive metabolites come from the flavonoid class based on metabolome data. The (), (), and () genes may be the key genes for the accumulation of anthocyanins, flavonols, and flavans in response to drought stress in blueberry leaves, respectively. Delphinidin 3-glucoside and delphinidin-3-O-glucoside chloride may be the most important drought-responsive flavonoid metabolites. , , , and might be responsible for drought-induced flavonoid biosynthesis and , may be responsible for blueberry leaf drought tolerance. ABA responsive elements binding factor () genes, genes, genes, and flavonoid biosynthetic genes might form a regulatory network to regulate drought-induced accumulation of flavonoid metabolites in blueberry leaves. Our study provides a useful reference for breeding drought-resistant blueberry varieties.
蓝莓（ spp.）对干旱胁迫极为敏感。类黄酮是重要的次生代谢物，具有耐受干旱胁迫的能力。因此，通过增加类黄酮含量来提高蓝莓的抗旱性对于蓝莓产业的发展至关重要。为了探索蓝莓适应干旱胁迫的潜在分子机制，我们对蓝莓叶片在干旱胁迫下的代谢组和转录组进行了综合分析。我们发现，基于转录组数据，最丰富的干旱响应基因主要参与类黄酮生物合成和植物激素信号转导途径；基于代谢组数据，主要的干旱响应代谢物来自类黄酮类。 （）、 （）和 （）基因可能分别是蓝莓叶片响应干旱胁迫积累花青素、类黄酮和黄烷醇的关键基因。飞燕草素 3-葡萄糖苷和飞燕草素-3-O-葡萄糖苷氯化物可能是最重要的干旱响应类黄酮代谢物。 、 、 、和 可能负责干旱诱导的类黄酮生物合成， 可能负责蓝莓叶片的耐旱性。脱落酸响应元件结合因子（）基因、 基因、 基因和类黄酮生物合成基因可能形成一个调控网络，调节干旱诱导的蓝莓叶片类黄酮代谢物的积累。我们的研究为培育抗旱性蓝莓品种提供了有益的参考。
