Fatima Rida, Batool Aqsa, Ijaz Tabinda, Khan Sajjad Ullah, Akhtar Yasmin, Ercisli Sezai, Almutairi Saeedah Musaed, Elshikh Mohamed Soliman, Saleem Aroona, Javed Muhammad Ammar
Faculty of Allied Health Sciences, The Superior University, Lahore, 55150 Pakistan.
Department of Chemistry, Government College University, Lahore, 54000 Pakistan.
Cytotechnology. 2025 Feb;77(1):36. doi: 10.1007/s10616-024-00663-w. Epub 2025 Jan 5.
Homeostasis of tissues requires a complex balance between cell proliferation and cell death. The disruption of this balance leads to tumors. Cancer is a mortal disease that spreads all over the body, it is an irregular cell growth. All the tumors are not cancerous, benign tumors do not spread to all body parts. Cancer is caused when cells start to grow out of control, known as increased proliferation. Cancer is of many kinds and the reason for cancer is the uncontrolled growth of cells that can affect any tissue of the body Conventional plants are precious sources of novel cytotoxic agents and are still in a better role in health concerns. The study aimed to evaluate the effect of in vitro anti-proliferative activity of plant extracts against HeLa and HepG2 cell lines and compared with normal BHK cells. plant (seed, leaves, fruit peel, fruit pulp, stem, and root) is extracted in their solvent's ethanol, ethyl acetate, and petroleum ether. For the estimation of anti-proliferation, cell viability, and cell death in HeLa, HepG2, BHK, MTT assay was done. The angiogenic potential was checked via Immunocytochemistry and ELISA of VEGF. Immunocytochemistry and ELISA of Annexin-V were performed for the estimation of apoptosis in HeLa cells and BHK. Furthermore, Immunocytochemistry and ELISA for p53 were also performed. Cancer cells (HeLa, HepG2) showed reduced angiogenesis, low proliferation, increased apoptotic level, reduced viability, and increased cell death. It is found that plant extract induces apoptosis along with inhibition of proliferation and angiogenesis may strongly have profound effects on growth suppression of HeLa and HepG2 cell lines.
组织的稳态需要细胞增殖和细胞死亡之间的复杂平衡。这种平衡的破坏会导致肿瘤。癌症是一种致命疾病，会扩散至全身，是一种异常的细胞生长。并非所有肿瘤都是癌性的，良性肿瘤不会扩散到身体的所有部位。当细胞开始失控生长，即增殖增加时，就会引发癌症。癌症有多种类型，其病因是细胞的不受控制生长，可影响身体的任何组织。传统植物是新型细胞毒性剂的宝贵来源，在健康问题方面仍发挥着重要作用。该研究旨在评估植物提取物对HeLa和HepG2细胞系的体外抗增殖活性，并与正常BHK细胞进行比较。将植物（种子、叶子、果皮、果肉、茎和根）在乙醇、乙酸乙酯和石油醚溶剂中进行提取。为了评估HeLa、HepG2、BHK细胞中的抗增殖、细胞活力和细胞死亡情况，进行了MTT试验。通过免疫细胞化学和VEGF的ELISA检测血管生成潜力。对HeLa细胞和BHK细胞进行Annexin-V的免疫细胞化学和ELISA检测以评估细胞凋亡。此外，还进行了p53的免疫细胞化学和ELISA检测。癌细胞（HeLa、HepG2）显示出血管生成减少、增殖降低、凋亡水平增加、活力降低和细胞死亡增加。研究发现，植物提取物可诱导细胞凋亡，同时抑制增殖和血管生成，可能对HeLa和HepG2细胞系的生长抑制产生深远影响。
