Yu Fei, Zhao Xing, Zhang Shuting, Lu Wenxin, Li Peilin, Yang Wei, Zhao Zhihe
State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
Department of Nephrology, Kidney Research Institute, West China Hospital of Sichuan University, Chengdu, 610041, China.
Adv Healthc Mater. 2025 Mar;14(6):e2404015. doi: 10.1002/adhm.202404015. Epub 2025 Jan 7.
Immune-mediated bone regeneration driven by bone biomaterials offers a therapeutic strategy for repairing bone defects. Among 2D nanomaterials, TiCT MXenes have garnered substantial attention for their potential in tissue regeneration. This investigation concentrates on the role of MXene nanocomposites in modulating the immune microenvironment within bone defects to facilitate bone tissue restoration. TiCT MXenes are synthetized, incorporated into beta-tricalcium phosphate ceramics (β-TCP) nanocomposites (T-MXene), and their osteoinductive and immunomodulatory effects are evaluated. The effects of T-MXene-treated T-cells on bone marrow stromal cells (BMSCs) are explored. In addition, its therapeutic potential for bone regeneration is assessed in vivo using a critical-sized mandibular bone defect model. The underlying mechanisms by which T-MXene regulates T-cell differentiation and bone regeneration are explored via whole-transcriptome RNA sequencing. The scaffolds activate N-glycosylation in T cells, which possess anti-inflammatory and antioxidant effects, thereby inducing a pro-regenerative response. T-MXene increased the proportion of IL-4 T cells among primary T cells and mandibular lymph nodes, ultimately promoting osteogenesis in BMSCs and injured mandibles. The distinctive function of MXene-based nanocomposites in osteoimmunomodulation provides a solid foundation for further exploration and application of MXenes as immune response modulators, potentially advancing their use in regenerative medicine.
由骨生物材料驱动的免疫介导骨再生为修复骨缺损提供了一种治疗策略。在二维纳米材料中，TiCT MXene因其在组织再生方面的潜力而备受关注。本研究集中于MXene纳米复合材料在调节骨缺损内免疫微环境以促进骨组织修复中的作用。合成TiCT MXene，将其掺入β-磷酸三钙陶瓷（β-TCP）纳米复合材料（T-MXene）中，并评估其骨诱导和免疫调节作用。探讨了经T-MXene处理的T细胞对骨髓间充质干细胞（BMSC）的影响。此外，使用临界大小的下颌骨缺损模型在体内评估其骨再生的治疗潜力。通过全转录组RNA测序探索T-MXene调节T细胞分化和骨再生的潜在机制。这些支架激活T细胞中的N-糖基化，具有抗炎和抗氧化作用，从而诱导促再生反应。T-MXene增加了原代T细胞和下颌淋巴结中IL-4 T细胞的比例，最终促进了BMSC和成骨损伤下颌骨的成骨作用。基于MXene的纳米复合材料在骨免疫调节中的独特功能为进一步探索和应用MXene作为免疫反应调节剂提供了坚实基础，有望推动其在再生医学中的应用。
