Li Chuang, Zhang Butian, Kim Minkyu, Liu Haixing, Yang Feiyang, Chen Ke, Shi Hongfeng
China-Japan Union Hospital of Jilin University, Rehabilitation Medicine Department, Changchun, Jilin, China; Division of Animal and Dairy Science, College of Agriculture and Life Science, Chungnam National University, Daejeon, South Korea.
China-Japan Union Hospital of Jilin University, Rehabilitation Medicine Department, Changchun, Jilin, China.
Anim Reprod Sci. 2025 Feb;273:107761. doi: 10.1016/j.anireprosci.2024.107761. Epub 2024 Dec 29.
Assisted reproductive technology (ART) is widely used to address infertility and enhance reproductive outcomes in livestock. Among various ART techniques, in vitro maturation (IVM) is commonly used to obtain high-quality oocytes but is susceptible to oxidative stress. In traditional Chinese medicine, Rhizoma Atractylodis Macrocephalae (Bai Zhu) is used to enhance maternal and fetal health. Atractylenolide Ⅲ (AⅢ), a major component of Bai Zhu, has shown both antioxidant properties and oxidative stress induction, leading to controversy. This study used porcine oocytes as a model to investigate the effects of AⅢ under tunicamycin (TM)-induced oxidative stress. During IVM, oocytes were treated with various concentrations of AⅢ and a constant dose of TM. AⅢ promoted oocyte maturation and cumulus cell expansion, with the optimal concentration being 1 mg/L. AⅢ reduced reactive oxygen species (ROS) and malondialdehyde (MDA) levels, indicating reduced oxidative damage. Mitochondrial function and membrane potential (MMP) were preserved in AⅢ-treated oocytes. Additionally, AIII could alleviate TM-induced endoplasmic reticulum (ER) stress, as shown by decreased mRNA expression of ER stress markers. Following parthenogenetic activation (PA), AⅢ-treated oocytes exhibited increased cleavage and blastocyst formation rates with reduced apoptosis compared to the TM group. These findings suggest that AⅢ protects against oxidative stress, improving oocyte quality and developmental potential, with potential applications in ART.
辅助生殖技术（ART）被广泛用于解决家畜不育问题并提高繁殖效率。在各种ART技术中，体外成熟（IVM）常用于获取高质量的卵母细胞，但易受氧化应激影响。在传统中医中，白术用于增强母婴健康。白术的主要成分苍术酮Ⅲ（AⅢ）既具有抗氧化特性，又能诱导氧化应激，引发了争议。本研究以猪卵母细胞为模型，研究AⅢ在衣霉素（TM）诱导的氧化应激下的作用。在IVM过程中，用不同浓度的AⅢ和恒定剂量的TM处理卵母细胞。AⅢ促进了卵母细胞成熟和卵丘细胞扩展，最佳浓度为1mg/L。AⅢ降低了活性氧（ROS）和丙二醛（MDA）水平，表明氧化损伤减少。AⅢ处理的卵母细胞中线粒体功能和膜电位（MMP）得以保留。此外，AⅢ可减轻TM诱导的内质网（ER）应激，表现为ER应激标志物的mRNA表达降低。孤雌激活（PA）后，与TM组相比，AⅢ处理的卵母细胞的卵裂率和囊胚形成率增加，凋亡减少。这些发现表明，AⅢ可抵御氧化应激，提高卵母细胞质量和发育潜力，在ART中具有潜在应用价值。
