Lee Seunghyung
College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea.
Animals (Basel). 2025 May 14;15(10):1422. doi: 10.3390/ani15101422.
Tunicamycin induces endoplasmic reticulum stress in mammalian cells. Our study aimed to investigate the effect of tunicamycin on the motility and viability of sperm, reactive oxygen species, nitric oxide, and lipid peroxidation in boar sperm. We treated 1.0, 2.0, 5.0, and 10 μM of tunicamycin in boar semen, and experimental treatments were performed. The viability (55.44%, 53.20, and 40.00%, < 0.05) and motility (73.28%, 71.48%, and 54.48%, < 0.05) of sperm at 2.0, 5.0, and 10.0 μM were decreased by tunicamycin, and the levels of reactive oxygen species and lipid peroxidation in tunicamycin-treated boar semen were increased ( < 0.05). However, the nitric oxide level was not changed by tunicamycin. Based on the results, we indicated that tunicamycin induces cell death by increasing oxidative stress in boar sperm, which may be the cause of decreased sperm viability and motility. Thus, we suggest that tunicamycin may induce cell death due to oxidative stress by reactive oxygen species and lipid peroxidation.
衣霉素可诱导哺乳动物细胞内质网应激。我们的研究旨在探讨衣霉素对猪精子活力、生存能力、活性氧、一氧化氮及脂质过氧化的影响。我们在猪精液中分别加入1.0、2.0、5.0和10 μM的衣霉素，并进行实验处理。2.0、5.0和10.0 μM衣霉素处理组的精子生存能力（分别为55.44%、53.20%和40.00%，P<0.05）和活力（分别为73.28%、71.48%和54.48%，P<0.05）均降低，且衣霉素处理的猪精液中活性氧和脂质过氧化水平升高（P<0.05）。然而，衣霉素对一氧化氮水平无影响。基于这些结果，我们指出衣霉素通过增加猪精子的氧化应激诱导细胞死亡，这可能是精子活力和生存能力下降的原因。因此，我们认为衣霉素可能由于活性氧和脂质过氧化导致的氧化应激而诱导细胞死亡。
