Lee Hayoung, Kim Hyewon, An Jisoon, Cheong Hee-Tae, Lee Sang-Hee
College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea.
College of Veterinary Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea.
Animals (Basel). 2024 Dec 10;14(24):3562. doi: 10.3390/ani14243562.
Pig production through crossbreeding methods is a pillar of the swine industry; however, research on the fertilization ability of male pigs in crossbreeds is lacking. Therefore, this study investigated the effects of Duroc sperm (DS) and Landrace sperm (LS) on fertility in Yorkshire × Landrace × Duroc (YLD) oocytes. Sperm were collected from the Duroc and Landrace species, and sperm characteristics, viability, and acrosome reactions were analyzed using flow cytometry. Oocytes were collected from YLD ovaries, and the fertility of DS and LS was determined using in vitro fertilization (IVF). Reactive oxygen species (ROS) and antioxidative abilities were analyzed using HDCFDA and a Cell Tracker Red assay. Pluripotency (, , and , antioxidative (, , , and ), apoptotic ( and ), and cell cycle-related ( and ) genes were detected using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in oocytes fertilized with sperm. The results showed no significant difference in viability or acrosome reaction between DS and LS. ROS levels were significantly lower in the LS group than in the DS group, whereas glutathione (GSH) levels in the embryo did not significantly differ between the DS and LS groups. The , , and mRNA expression levels were significantly higher in the LS than DS groups. Blastocyst formation was significantly higher in the LS than DS groups. ROS levels were reduced, and blastocyte formation was increased in LS-obtained embryos. In conclusion, these results provide a fundamental understanding of using Landrace semen in the three-way crossbreeding of YLD pigs.
通过杂交方法进行生猪生产是养猪业的支柱；然而，关于杂交公猪受精能力的研究却很缺乏。因此，本研究调查了杜洛克精子（DS）和长白猪精子（LS）对约克夏×长白×杜洛克（YLD）卵母细胞受精能力的影响。从杜洛克和长白猪品种采集精子，使用流式细胞术分析精子特征、活力和顶体反应。从YLD卵巢收集卵母细胞，使用体外受精（IVF）确定DS和LS的受精能力。使用HDCFDA和细胞追踪红分析法分析活性氧（ROS）和抗氧化能力。在精子受精的卵母细胞中，使用定量逆转录聚合酶链反应（qRT-PCR）检测多能性（、、和）、抗氧化（、、、和）、凋亡（和）以及细胞周期相关（和）基因。结果显示，DS和LS在活力或顶体反应方面没有显著差异。LS组的ROS水平显著低于DS组，而DS组和LS组胚胎中的谷胱甘肽（GSH）水平没有显著差异。LS组的、和mRNA表达水平显著高于DS组。LS组的囊胚形成率显著高于DS组。在LS获得的胚胎中，ROS水平降低，胚泡形成增加。总之，这些结果为在YLD猪三元杂交中使用长白猪精液提供了基本认识。
