Yu Cheng-Dong, Kim Sang-Won, Liu Cun-Xia, Gao Yue-Hua, Li Yu-Feng, Park Jong-Yeol, Cha Se-Yeoun, Jang Hyung-Kwan, Kang Min, Wei Bai
Department of Avian Diseases, College of Veterinary Medicine and Center for Avian Disease, Jeonbuk National University, Iksan 54596, Republic of Korea.
Institute of Poultry Science, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Livestock and Poultry Breeding, Jinan 250100, China.
Animals (Basel). 2024 Dec 16;14(24):3630. doi: 10.3390/ani14243630.
Duck circovirus (DuCV) infections cause immunosuppression in ducks, potentially leading to significant economic losses for the duck farming industry. This study investigates the prevalence, genetic characteristics, and evolutionary trends of DuCV in Korea between 2013 and 2022. Samples from 184 farms across seven provinces were analyzed using polymerase chain reaction (PCR). The overall DuCV infection rate was 29.4% (54/184), with Jeollanam-do showing the highest prevalence (37.5%, 15/40). Ducks aged 3-6 weeks were most susceptible to infection, while ducklings younger than one week were rarely infected. Whole-genome sequencing was performed on 24 positive samples with phylogenetic analysis revealing that DuCV-1b is the predominant subtype in Korea (23/24 strains). Notably, a Korean DuCV-1a subtype strain was identified for the first time, showing close genetic relatedness to Chinese DuCV 1a strains. Novel subtype-specific amino acid variations in ORF1 and ORF2 were statistically analyzed and classified. Recombination analysis suggested some Korean DuCV-1b strains may have resulted from recombination events involving strains from different countries. This comprehensive study provides crucial insights into the current prevalence, genetic diversity, and evolutionary dynamics of DuCV in Korea, offering valuable data for developing effective control strategies and understanding the global epidemiology of this economically important avian pathogen.
鸭圆环病毒（DuCV）感染会导致鸭的免疫抑制，可能给养鸭业造成重大经济损失。本研究调查了2013年至2022年期间韩国DuCV的流行情况、遗传特征和进化趋势。使用聚合酶链反应（PCR）对来自七个省份184个养殖场的样本进行了分析。DuCV的总体感染率为29.4%（54/184），全罗南道的患病率最高（37.5%，15/40）。3至6周龄的鸭最易感染，而1周龄以下的雏鸭很少感染。对24个阳性样本进行了全基因组测序，系统发育分析表明DuCV-1b是韩国的主要亚型（23/24株）。值得注意的是，首次鉴定出一株韩国DuCV-1a亚型毒株，它与中国的DuCV 1a毒株显示出密切的遗传关系。对ORF1和ORF2中新型亚型特异性氨基酸变异进行了统计分析和分类。重组分析表明，一些韩国DuCV-1b毒株可能是由涉及不同国家毒株的重组事件导致的。这项全面的研究为了解韩国DuCV的当前流行情况、遗传多样性和进化动态提供了关键见解，为制定有效的控制策略和理解这种具有经济重要性的禽类病原体的全球流行病学提供了有价值的数据。
