Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Trau Quy Town, Gia Lam District, Hanoi, 131000, Vietnam.
National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi, Vietnam.
Arch Virol. 2022 Sep;167(9):1871-1877. doi: 10.1007/s00705-022-05501-y. Epub 2022 Jun 18.
In the present study, tissue samples collected from 130 ducks from clinically suspected commercial flocks and diseased birds in six provinces of northern Vietnam were tested for duck circovirus (DuCV) infection. The DuCV genome was detected in 56 out of 130 (43.08%) duck samples by PCR. Of 38 tested farms, 26 (68.42%) were positive for the DuCV genome. The rate of the DuCV genome detection in ducks at 3-4 weeks of age (54.17%) was significantly higher (p < 0.05) than that at <3 (32.43%) and >7 (33.33%) weeks of age and insignificantly higher than that at 5-7 weeks of age (43.33%) (p = 0.11). The genomes of six Vietnamese DuCV isolates were determined. They ranged in length from 1,988 to 1,995 nucleotides, and their nucleotide sequences were 83.24% to 99.69% identical to each other. Phylogenetic analysis based on the complete genome sequences indicated that the DuCV strains circulating in northern Vietnam can be divided into two main genotypes (I and II) and several subgenotypes. The Vietnamese DuCV isolates were closely related to Chinese, Taiwanese, and Korean strains. One positively selected site was detected in the capsid protein.
在本研究中，从越南北部六个省的临床疑似商业禽类和患病禽类中采集了 130 只鸭的组织样本，检测鸭圆环病毒（DuCV）感染情况。通过 PCR 检测到 130 份鸭样本中有 56 份（43.08%）存在 DuCV 基因组。在 38 个检测农场中，有 26 个（68.42%）检测到 DuCV 基因组阳性。3-4 周龄鸭的 DuCV 基因组检出率（54.17%）显著高于<3 周龄（32.43%）和>7 周龄（33.33%）鸭（p<0.05），与 5-7 周龄鸭（43.33%）的检出率无显著差异（p=0.11）。测定了六个越南 DuCV 分离株的基因组。它们的长度从 1988 到 1995 个核苷酸不等，核苷酸序列彼此之间的相似度为 83.24%到 99.69%。基于全基因组序列的系统进化分析表明，在越南北部流行的 DuCV 株可分为两个主要基因型（I 和 II）和几个亚型。越南 DuCV 分离株与中国、中国台湾和韩国的分离株密切相关。在衣壳蛋白中检测到一个正选择位点。
