Asir Berna, Kumtepe Yakup
Department of Obstetrics and Gynecology, Erzurum City Hospital, Erzurum 25030, Turkey.
Department of Obstetrics and Gynecology, Ankara University, Ankara 06100, Turkey.
Animals (Basel). 2024 Dec 20;14(24):3674. doi: 10.3390/ani14243674.
This study aimed to investigate the effect of antihypertensive drugs on reproductive function in Rattus norvegicus and demonstrate the potential role of oxidative stress in reproductive dysfunction. Rattus norvegicus were selected as the experimental animals and divided into the following groups: healthy (control group), clonidine (CL), rilmenidine (RLD), methyldopa (MTL), amlodipine (ALD), and ramipril (RML). Each individual in each group was marked from one to six. Doses of clonidine (0.075 mg/kg), rilmenidine (0.5 mg/kg), methyldopa (100 mg/kg), amlodipine (2 mg/kg), and ramipril (2 mg/kg) were administered orally via gavage to each Rattus norvegicus. Using blood obtained from Rattus norvegicus, the absorbance of the pink-colored complex formed by thiobarbituric acid (TBA) and malondialdehyde (MDA) was measured spectrophotometrically at the 532 nm wavelength. Blood samples were collected from the tail veins to analyze serum malondialdehyde (MDA) and total glutathione levels in the serum of all Rattus norvegicus. After sampling, two mature male Rattus norvegicus were introduced to every group of six female Rattus norvegicus and accommodated in a controlled laboratory environment for two months. Any female Rattus norvegicus that became pregnant during this time was transferred to a solitary cage within a controlled setting. Rattus norvegicus that did not become pregnant and did not give birth during this period were considered infertile. The results were compared among the groups. Total glutathione (tGSH) levels were determined using a spectrophotometer. According to our study, the increase in MDA levels observed was not statistically significant in the CL and RLD groups compared to that in the control group. MDA levels were significantly increased in the methyldopa, amlodipine, and RML groups. While total glutathione levels in the CL group were similar to those in the control group, the RLD, MTL, ALD, and RML groups showed a statistically significant decrease. While the animals in the CL and RLD groups were not infertile, infertility was apparent in the groups treated with MTL, ALD, and RML. Thus, it was determined that the antihypertensive drugs MTL, ALD, and RML had different effects on fertility, and that the use of such drugs could cause infertility by increasing oxidative stress and decreasing antioxidant levels.
本研究旨在探讨抗高血压药物对褐家鼠生殖功能的影响，并证明氧化应激在生殖功能障碍中的潜在作用。选用褐家鼠作为实验动物，分为以下几组：健康组（对照组）、可乐定（CL）组、利美尼定（RLD）组、甲基多巴（MTL）组、氨氯地平（ALD）组和雷米普利（RML）组。每组中的每只动物都从1到6进行标记。将可乐定（0.075mg/kg）、利美尼定（0.5mg/kg）、甲基多巴（100mg/kg）、氨氯地平（2mg/kg）和雷米普利（2mg/kg）的剂量通过灌胃法口服给予每只褐家鼠。使用从褐家鼠采集的血液，在532nm波长下用分光光度计测量硫代巴比妥酸（TBA）与丙二醛（MDA）形成的粉红色复合物的吸光度。从尾静脉采集血样，分析所有褐家鼠血清中的丙二醛（MDA）和总谷胱甘肽水平。采样后，将两只成熟雄性褐家鼠放入每组六只雌性褐家鼠中，并在可控的实验室环境中饲养两个月。在此期间怀孕的任何雌性褐家鼠都被转移到可控环境中的单独笼子里。在此期间未怀孕且未分娩的褐家鼠被视为不育。对各组结果进行比较。使用分光光度计测定总谷胱甘肽（tGSH）水平。根据我们的研究，与对照组相比，CL组和RLD组中观察到的MDA水平升高无统计学意义。甲基多巴、氨氯地平和RML组的MDA水平显著升高。虽然CL组的总谷胱甘肽水平与对照组相似，但RLD、MTL、ALD和RML组显示出统计学上的显著下降。虽然CL组和RLD组的动物没有不育，但MTL、ALD和RML治疗组出现了不育现象。因此，确定抗高血压药物MTL、ALD和RML对生育能力有不同影响，并且使用这些药物可能通过增加氧化应激和降低抗氧化剂水平导致不育。
