Tan Wenting, Tang Yiwei, Liu Fang, Lu Li, Liu An, Ye Haihui
State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, China.
Animals (Basel). 2024 Dec 23;14(24):3706. doi: 10.3390/ani14243706.
In this study, we identified and its putative receptor from the mud crab and explored its potential role in ovarian development. RT-PCR results suggested was extensively expressed in nervous tissues, the ovary, the middle gut, and the Y-organ, while was highly expressed in the ovary. The expression level of in the ovary, eyestalk, and cerebral ganglia gradually increased during ovarian development, whereas its receptor exhibited an opposite expression pattern in the ovary. Immunofluorescence revealed that ACP was specifically localized in the follicle cells of the ovary. In vitro experiments showed that the expression of vitellogenin receptor () in the ovary was significantly increased by 4 and 6 h incubation of -ACP (10 nM). In addition, 12 h injection of -ACP significantly induced the levels of in the hepatopancreas and in the ovary, and hemolymph 17β-estradiol titer. Finally, it demonstrated that prolonged injection of -ACP significantly increased the level of and expression, hemolymph 17β-estradiol titer, GSI, and the oocyte diameter. In conclusion, our results suggested that ACP is involved in the regulation of ovarian development of , likely by inducing hepatopancreas expression through estradiol and promoting the uptake of by oocytes.
在本研究中，我们从青蟹中鉴定出 及其假定受体，并探讨了其在卵巢发育中的潜在作用。逆转录聚合酶链反应（RT-PCR）结果表明， 在神经组织、卵巢、中肠和Y器官中广泛表达，而 在卵巢中高表达。 在卵巢、眼柄和脑神经节中的表达水平在卵巢发育过程中逐渐升高，而其受体在卵巢中呈现相反的表达模式。免疫荧光显示，酸性磷酸酶（ACP）特异性定位于卵巢的卵泡细胞中。体外实验表明，用10 nM的 -ACP孵育4小时和6小时后，卵巢中卵黄蛋白原受体（ ）的表达显著增加。此外，注射 -ACP 12小时可显著诱导肝胰腺中 的水平以及卵巢中 和血淋巴17β-雌二醇滴度。最后，结果表明，长时间注射 -ACP可显著提高 的水平和 表达、血淋巴17β-雌二醇滴度、性腺指数（GSI）和卵母细胞直径。总之，我们的结果表明，ACP可能通过雌二醇诱导肝胰腺 表达并促进卵母细胞对 的摄取，从而参与青蟹卵巢发育的调控。
