Choi Da-In, Zayed Mohammed, Na Eun-Jee, Oem Jae-Ku, Jeong Byung-Hoon
Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Republic of Korea.
Department of Bioactive Material Sciences, Institute for Molecular Biology and Genetics, Jeonbuk National University, Jeonju 54896, Republic of Korea.
Animals (Basel). 2024 Dec 23;14(24):3716. doi: 10.3390/ani14243716.
Prion diseases, or transmissible spongiform encephalopathies (TSEs), are a group of invariably fatal neurodegenerative disorders. One of the candidate genes involved in prion diseases is the shadow of the prion protein () gene. Raccoon dogs, a canid, are considered to be a prion disease-resistant species. To date, the genetic polymorphisms of the gene and the predicted protein structure of the shadow of prion protein (Sho) have not been explored in raccoon dogs. was amplified using polymerase chain reaction (PCR). We also investigated the genetic polymorphisms of by analyzing the frequencies of genotypes, alleles, and haplotypes, as well as the linkage disequilibrium among the identified genetic variations. In addition, analysis with MutPred-Indel was performed to predict the pathogenicity of insertion/deletion polymorphisms. Predicted 3D structures were analyzed by the Alphafold2. We found a total of two novel synonymous single nucleotide polymorphisms and three insertion/deletion polymorphisms. In addition, the 3D structure of the Sho protein in raccoon dogs was predicted to resemble that of the Sho protein in dogs. This is the first study regarding the genetic and structural characteristics of the raccoon dog gene.
朊病毒病，即传染性海绵状脑病（TSEs），是一组必然致命的神经退行性疾病。参与朊病毒病的候选基因之一是朊病毒蛋白（）基因的影子基因。貉，一种犬科动物，被认为是对朊病毒病有抗性的物种。迄今为止，尚未在貉中探索该基因的遗传多态性以及朊病毒蛋白影子（Sho）的预测蛋白质结构。使用聚合酶链反应（PCR）扩增该基因。我们还通过分析基因型、等位基因和单倍型的频率以及已鉴定遗传变异之间的连锁不平衡来研究该基因的遗传多态性。此外，使用MutPred-Indel进行分析以预测插入/缺失多态性的致病性。通过Alphafold2分析预测的三维结构。我们总共发现了两个新的同义单核苷酸多态性和三个插入/缺失多态性。此外，预测貉中Sho蛋白的三维结构与狗中Sho蛋白的三维结构相似。这是关于貉基因遗传和结构特征的首次研究。
