Wang Jiaxue, An Wei, Wang Zhenlong, Zhao Ya, Han Bing, Tao Hui, Wang Jinquan, Wang Xiumin
Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Beijing 100081, China.
Antioxidants (Basel). 2024 Dec 17;13(12):1544. doi: 10.3390/antiox13121544.
A large number of cases of infectious colitis caused by multidrug-resistant (MDR) bacteria, such as , can result in colon damage and severe inflammation. Vanilla, a widely utilized flavor and fragrance compound, is extensively used in various food. However, the effect of vanilla on MDR -induced infectious colitis has received less attention. In this study, the antibacterial activity of vanillin against MDR and other bacteria was determined by the microtiter broth dilution method. The antioxidant and anti-inflammatory capacity of vanillin was assessed in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and MDR -induced mouse colitis. The results demonstrated that vanillin exhibited potent antibacterial activity against various strains of MDR , , and , with a minimal inhibitory concentration (MIC) of 1.25-2.5 mg/mL and a minimum bactericidal concentration (MBC) of 5-10 mg/mL; it effectively inhibited cell division in . Vanillin also displayed remarkable antioxidant activity by suppressing the levels of malondialdehyde (MDA), superoxide dismutase (SOD), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 cell; it significantly reduced the production of inflammatory mediators including nitroxide (NO), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β), while increasing interleukin 10 (IL-10). In an MDR -induced mouse colitis model, vanillin effectively inhibited inflammation by suppressing inflammatory cytokines, mitogen-activated protein kinase (MAPK), and nuclear factor κ-B (NF-κB) cell signaling pathway activation; it ameliorated changes in intestinal microflora characterized by decreased Firmicutes richness alongside increased Bacteroides richness, rebalancing the dysbiosis caused by . These findings highlight the potential pharmacological applicability of vanillin as a promising bioactive molecule for treating infectious colitis.
大量由多重耐药（MDR）细菌引起的感染性结肠炎病例，比如（此处原文未提及具体细菌名称），可导致结肠损伤和严重炎症。香草醛是一种广泛使用的香料化合物，被广泛应用于各类食品中。然而，香草醛对多重耐药菌诱导的感染性结肠炎的影响却较少受到关注。在本研究中，采用微量肉汤稀释法测定了香草醛对多重耐药菌及其他细菌的抗菌活性。在脂多糖（LPS）刺激的RAW 264.7细胞和多重耐药菌诱导的小鼠结肠炎模型中评估了香草醛的抗氧化和抗炎能力。结果表明，香草醛对多种多重耐药菌菌株（此处原文未提及具体细菌名称）具有强大的抗菌活性，最低抑菌浓度（MIC）为1.25 - 2.5毫克/毫升，最低杀菌浓度（MBC）为5 - 10毫克/毫升；它能有效抑制（此处原文未提及具体细菌名称）中的细胞分裂。香草醛还通过抑制LPS刺激的RAW 264.7细胞中丙二醛（MDA）、超氧化物歧化酶（SOD）和活性氧（ROS）的水平表现出显著的抗氧化活性；它显著降低了包括一氧化氮（NO）、肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）和白细胞介素1β（IL-1β）在内的炎症介质的产生，同时增加了白细胞介素10（IL-10）。在多重耐药菌诱导的小鼠结肠炎模型中，香草醛通过抑制炎性细胞因子、丝裂原活化蛋白激酶（MAPK）和核因子κB（NF-κB）细胞信号通路的激活有效抑制了炎症；它改善了肠道微生物群的变化，其特征为厚壁菌门丰度降低，拟杆菌门丰度增加，从而重新平衡了由（此处原文未提及具体细菌名称）引起的生态失调。这些发现突出了香草醛作为一种有前景的生物活性分子在治疗感染性结肠炎方面潜在的药理学适用性。
