Department of Chemistry, Faculty of Science, Polymer Research Group, University of Tanta, Tanta, Egypt.
Department of Chemistry, College of Science, King Faisal University, Al-Ahsa, Saudi Arabia.
J Appl Biomater Funct Mater. 2024 Jan-Dec;22:22808000241281273. doi: 10.1177/22808000241281273.
Vanillin loaded-physically crosslinked hydrogel membranes made of PVA/chitosan/itaconic acid (PVA-CS-IA) were prepared using freezing-thawing (F-T) cycle method. To ensure the entanglement of PVA-CS-IA chains, three F-T cycles were repeated. The polymeric chains entanglements were confirmed and characterized by different instrumental characterizations. Physicochemical properties for example, swelling ratio, mechanical characteristics, gel fraction percentage (GF%), hydrolytic degradation, and thermal stability of PVA-CS-IA membrane were discussed in detail. The findings showed that the swelling ratio, mechanical characteristics, and hydrolytic degradation of the crosslinked membranes enhanced with increasing CS-IA contents in membranes composition; however, GF% gradually declined with CS-IA content. Additionally, cell viability test using HFB-4 cell line and antimicrobial activity against and were evaluated using MTT assay and the bacterium growth inhibition percentage method; respectively. Notably, with varying incubation durations and membrane concentrations, all examined constructed hydrogels showed significant cell survival percentages. The findings supported the notion that produced hydrogel membranes might be used in a professional setting as antibacterial dressings or biomaterials for quick wound healing rate.
采用冷冻-解冻（F-T）循环法制备了由 PVA/壳聚糖/衣康酸（PVA-CS-IA）组成的负载香草醛的物理交联水凝胶膜。为了确保 PVA-CS-IA 链的缠结，重复了三个 F-T 循环。通过不同的仪器特性确认和表征了聚合物链缠结。详细讨论了 PVA-CS-IA 膜的物理化学性质，例如溶胀比、机械性能、凝胶分数百分比（GF%）、水解降解和热稳定性。结果表明，随着膜组成中 CS-IA 含量的增加，交联膜的溶胀比、机械性能和水解降解能力增强；然而，GF% 逐渐随 CS-IA 含量的增加而下降。此外，使用 HFB-4 细胞系评估了细胞活力测试，使用 MTT 测定法和细菌生长抑制百分比法评估了对 和 的抗菌活性；分别。值得注意的是，随着孵育时间和膜浓度的变化，所有检查过的水凝胶都显示出显著的细胞存活率。研究结果表明，所制备的水凝胶膜可在专业环境中用作抗菌敷料或用于快速伤口愈合的生物材料。
