Yang Zhihe, Khan Sadaf Aiman, Walsh Laurence J, Ziora Zyta M, Seneviratne Chaminda Jayampath
School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, QLD 4072, Australia.
Oral Health Centre, School of Dentistry, University of Queensland, Herston, QLD 4006, Australia.
Antibiotics (Basel). 2024 Dec 18;13(12):1228. doi: 10.3390/antibiotics13121228.
Biofilms are structured microbial communities that adhere to various abiotic and biotic surfaces, where organisms are encased in an exo-polysaccharide matrix. Organisms within biofilms use various mechanisms that help them resist external challenges, such as antibiotics, rendering them more resistant to drugs. Therefore, researchers have attempted to develop suitable laboratory models to study the physical features of biofilms, their resistance mechanisms against antimicrobial agents, and their gene and protein expression profiles. However, current laboratory models suffer from various limitations. In this comprehensive review, we have summarized the various designs that have been used for laboratory biofilm models, presenting their strengths and limitations. Additionally, we have provided insight into improving these models to more closely simulate real-life scenarios, using newly developed techniques in additive manufacturing, synthetic biology, and bioengineering.
生物膜是附着在各种非生物和生物表面的结构化微生物群落，其中的生物体被包裹在胞外多糖基质中。生物膜内的生物体利用各种机制来帮助它们抵御外部挑战，如抗生素，从而使它们对药物更具抗性。因此，研究人员试图开发合适的实验室模型来研究生物膜的物理特性、它们对抗菌剂的抗性机制以及它们的基因和蛋白质表达谱。然而，目前的实验室模型存在各种局限性。在这篇综述中，我们总结了用于实验室生物膜模型的各种设计，阐述了它们的优点和局限性。此外，我们利用增材制造、合成生物学和生物工程等新开发的技术，深入探讨了如何改进这些模型以更紧密地模拟现实场景。
