Kumar Sriram, Nguyen Anh Tuan, Goswami Subir, Ferracane Jack, Koley Dipankar
Department of Chemistry, Oregon State University, Corvallis, OR, USA.
Department of Restorative Dentistry, Oregon Health & Science University, Portland, OR, USA.
Sens Actuators B Chem. 2023 Feb 1;376(Pt A). doi: 10.1016/j.snb.2022.133034. Epub 2022 Nov 22.
Biofilms are complex three-dimensional microbial communities that adhere to a variety of surfaces and interact with their surroundings. Because of the dynamic nature of biofilm formation, establishing a uniform technique for quantifying and monitoring biofilm volume, shape, and features in real-time is challenging. Herein, we describe a noninvasive electrochemical impedance approach for real-time monitoring of dental plaque-derived multispecies biofilm growth on a range of substrates. A working equation relating electrochemical impedance to live biofilm volume has been developed that is applicable to all three surfaces examined, including glass, dental filling resin, and Ca-releasing resin composites. Impedance changes of 2.5, 35, 50, and 65% correlated to biofilm volumes of 0.10 ± 0.01, 16.9 ± 2.2, 29.7 ± 2.3, and 38.6 ± 2.8 μm/μm, respectively. We discovered that glass, dental filling resin, and Ca-releasing dental composites required approximately 3.5, 4.5, and 6 days, respectively, to achieve a 50% change in impedance. The local pH change at the biofilm-substrate interfaces also monitored with potentiometry pH microsensor, and pH change varied according to biofilm volume. This impedance-based technique can be a useful analytical method for monitoring the growth of biofilms on a variety of substrates in real-time. Therefore, this technique may be beneficial for examining antibacterial properties of novel biomaterials.
生物膜是复杂的三维微生物群落，它们附着在各种表面上并与周围环境相互作用。由于生物膜形成的动态性质，建立一种用于实时定量和监测生物膜体积、形状及特征的统一技术具有挑战性。在此，我们描述了一种非侵入性电化学阻抗方法，用于实时监测一系列底物上牙菌斑来源的多物种生物膜生长。已开发出一个将电化学阻抗与活生物膜体积相关联的工作方程，该方程适用于所研究的所有三种表面，包括玻璃、牙科填充树脂和钙释放树脂复合材料。阻抗变化2.5%、35%、50%和65%分别对应生物膜体积0.10±0.01、16.9±2.2、29.7±2.3和38.6±2.8μm/μm。我们发现，玻璃、牙科填充树脂和钙释放牙科复合材料分别需要约3.5天、4.5天和6天才能实现阻抗50%的变化。还使用电位pH微传感器监测了生物膜-底物界面处的局部pH变化，pH变化随生物膜体积而变化。这种基于阻抗的技术可以成为实时监测各种底物上生物膜生长的有用分析方法。因此，该技术可能有助于研究新型生物材料的抗菌性能。
