令人深感悲痛的生活事件通过诱导基因和基因发生突变而发挥致癌作用。
Deeply Saddening Life Events Play a Carcinogenic Role by Inducing Mutations in and Genes.
作者信息
Kutluhan Ahmet, Topak Osman Z, Akca Hakan, Tokgun Elvan, Ozdel Osman I, Yilmaz Sevda, Sungurtekin Ugur, Erdem Ergun, Yaren Arzu
机构信息
Department of Otolaryngology, Head and Neck Surgery, Pamukkale University School of Medicine, Denizli 20070, Turkey.
Department of Psychiarty, Pamukkale University School of Medicine, Denizli 20070, Turkey.
出版信息
Genes (Basel). 2024 Nov 28;15(12):1531. doi: 10.3390/genes15121531.
BACKGROUND/OBJECTIVES: In the past few decades, many studies have been conducted to find out that psychological stress and cancer are closely linked. Moreover, it was reported that stress can induce mutations in gene level. Therefore, in this study we want to examine a relationship between stressful life events, gene mutation and cancer.
METHODS
Stressful Life Experiences Screening (SLES), Hospital Anxiety and Depression Scale (HADS) and the Coping with Stress Style Scale (CSS) were applied to the participants to examine relationship between stress and cancer.
RESULTS
NGS results showed higher level of mutations accumulated on and genes in cancer patients who were exposed to stressful life events. The expression status of and genes on patients with or without cancer and several cancer cell lines demonstrated that both and mRNA levels were downregulated only in cancer patients and cancer cell lines but not in cancer free control groups. Re-created overexpression of the WT- and WT- extremely inhibited cellular growth, cellular invasion in cancer cell lines, tumor growth in xenograft model too.
CONCLUSIONS
Our results indicate that Stressful Life Experiences may induce cancer development by increased somatic mutations in and genes.
背景/目的：在过去几十年中，已经进行了许多研究以发现心理压力与癌症密切相关。此外，有报道称压力可在基因水平上诱导突变。因此，在本研究中，我们想要研究应激性生活事件、基因突变与癌症之间的关系。
方法
对参与者应用应激性生活经历筛查（SLES）、医院焦虑抑郁量表（HADS）和应对压力方式量表（CSS）来研究压力与癌症之间的关系。
结果
二代测序（NGS）结果显示，经历应激性生活事件的癌症患者中， 和 基因上积累的突变水平更高。对有或无癌症的患者以及几种癌细胞系中 和 基因的表达状态进行检测，结果表明，只有癌症患者和癌细胞系中的 和 信使核糖核酸（mRNA）水平下调，而在无癌对照组中未下调。野生型 和野生型 的重新过表达也极大地抑制了癌细胞系中的细胞生长、细胞侵袭以及异种移植模型中的肿瘤生长。
结论
我们的结果表明，应激性生活经历可能通过增加 和 基因中的体细胞突变来诱导癌症发生。
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