Safiullina Asiya K, Ernazarova Dilrabo K, Turaev Ozod S, Rafieva Feruza U, Ernazarova Ziraatkhan A, Arslanova Sevara K, Toshpulatov Abdulqahhor Kh, Oripova Barno B, Kudratova Mukhlisa K, Khalikov Kuvandik K, Iskandarov Abdulloh A, Khidirov Mukhammad T, Yu John Z, Kushanov Fakhriddin N
Institute of Genetics and Plant Experimental Biology Academy of Sciences of Uzbekistan, Tashkent 111208, Uzbekistan.
Department of Genetics, National University of Uzbekistan, Tashkent 100174, Uzbekistan.
Genes (Basel). 2024 Nov 28;15(12):1533. doi: 10.3390/genes15121533.
: The classification and phylogenetic relationships of L. landraces, despite their proximity to southern Mexico, remain unresolved. This study aimed to clarify these relationships using SSR markers and hybridization methods, focusing on subspecies and race differentiation within L. : Seventy polymorphic SSR markers (out of 177 tested) were used to analyze 141 alleles and calculate genetic distances among accessions. Phylogenetic relationships were determined using MEGA software (version 11.0.13) and visualized in a phylogenetic tree. ANOVA in NCSS 12 was used for statistical analysis. Over 1000 inter-race crosses were conducted to assess boll-setting rates. : Distinct phylogenetic patterns were identified between subspecies and races, correlating with boll-setting rates. , , and showed no significant increase in boll-setting rates in reciprocal crosses. Cultivars Omad and Bakht, as paternal parents, yielded higher boll-setting rates. and displayed high boll- and seed-setting rates as maternal parents but low rates as paternal parents. Additionally, phylogenetic analysis revealed a close relationship between cultivars 'Omad' and 'Bakht' with race , indicating their close evolutionary relationship. : Reciprocal differentiation characteristics of subspecies and races, particularly and , should be considered during hybridization for genetic and breeding studies. Understanding the phylogenetic relationships among taxa is crucial for exploring the genetic diversity of this economically important species.
尽管陆地棉地方品种与墨西哥南部接近，但其分类和系统发育关系仍未解决。本研究旨在利用SSR标记和杂交方法阐明这些关系，重点关注陆地棉亚种和种族的分化：使用177个测试SSR标记中的70个多态性SSR标记分析141个等位基因，并计算种质间的遗传距离。使用MEGA软件（版本11.0.13）确定系统发育关系，并在系统发育树中可视化。使用NCSS 12中的方差分析进行统计分析。进行了1000多次种间杂交以评估结铃率。：在亚种和种族之间鉴定出明显的系统发育模式，与结铃率相关。[具体品种名1]、[具体品种名2]和[具体品种名3]在正反交中结铃率没有显著增加。品种Omad和Bakht作为父本时，结铃率较高。[具体品种名4]和[具体品种名5]作为母本时，结铃和结籽率较高，但作为父本时则较低。此外，系统发育分析揭示了品种“Omad”和“Bakht”与[具体种族名]之间的密切关系，表明它们在进化上关系密切。：在遗传和育种研究的杂交过程中，应考虑陆地棉亚种和种族的正反交分化特征，特别是[具体亚种名1]和[具体亚种名2]。了解陆地棉分类群之间的系统发育关系对于探索这种经济重要物种的遗传多样性至关重要。
