Ijaz Babar, Zhao Nan, Kong Jie, Hua Jinping
Laboratory of Cotton Genetics, Genomics and Breeding/Key Laboratory of Crop Heterosis and Utilization of Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, China.
Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, China.
Front Plant Sci. 2019 Dec 11;10:1585. doi: 10.3389/fpls.2019.01585. eCollection 2019.
Genetic improvement in fiber quality is one of the main challenges for cotton breeders. Fiber quality traits are controlled by multiple genes and are classified as complex quantitative traits, with a negative relationship with yield potential, so the genetic gain is low in traditional genetic improvement by phenotypic selection. The availability of genomic sequences facilitates the development of high-throughput molecular markers, quantitative trait loci (QTL) fine mapping and gene identification, which helps us to validate candidate genes and to use marker assisted selection (MAS) on fiber quality in breeding programs. Based on developments of high density linkage maps, QTLs fine mapping, marker selection and omics, we have performed trait dissection on fiber quality traits in diverse populations of upland cotton. QTL mapping combined with multi-omics approaches such as, RNA sequencing datasets to identify differentially expressed genes have benefited the improvement of fiber quality. In this review, we discuss the application of molecular markers, QTL mapping and MAS for fiber quality improvement in upland cotton.
纤维品质的遗传改良是棉花育种者面临的主要挑战之一。纤维品质性状由多个基因控制,属于复杂的数量性状,与产量潜力呈负相关,因此在传统的表型选择遗传改良中遗传增益较低。基因组序列的可用性促进了高通量分子标记的开发、数量性状位点(QTL)的精细定位和基因鉴定,这有助于我们验证候选基因,并在育种计划中对纤维品质进行标记辅助选择(MAS)。基于高密度连锁图谱、QTL精细定位、标记选择和组学的发展,我们对陆地棉不同群体的纤维品质性状进行了性状剖析。QTL定位与RNA测序数据集等多组学方法相结合以鉴定差异表达基因,这有利于纤维品质的改良。在本综述中,我们讨论了分子标记、QTL定位和MAS在陆地棉纤维品质改良中的应用。