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濒危红鳍鱼的染色体水平组装与注释()。

Chromosome-Level Assembly and Annotation of the Endangered Red-Wing Fish ().

作者信息

Zhu Xiangyun, Luo Yanping, Ma Baoshan, Shen Qi, Zheng Xingyu, Xu Mei, Sheng Qiang, Wu Junjie

机构信息

Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.

Wuhan Design and Engineering College, Wuhan 430205, China.

出版信息

Genes (Basel). 2024 Nov 28;15(12):1536. doi: 10.3390/genes15121536.

DOI:10.3390/genes15121536
PMID:39766803
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11675437/
Abstract

The red-wing fish (), an endangered species native to Yunnan, is endemic to Chenghai Lake. The natural population of this species has suffered a sharp decline due to the invasion of alien fish species. Fortunately, the artificial domestication and reproduction of have been successful and this species has become an economic species locally. However, there is still little research on . In this study, a high-quality genome of was assembled and annotated. The genome was sequenced and assembled using the PacBio platform and Hi-C method. The genome size is 1.01 Gb and N50 is 37.99 Mb. The assembled contigs were anchored into 24 chromosomes. BUSCO analysis revealed that the genome assembly has 95.6% gene coverage completeness. A total of 455.62 Mb repeat sequences (48.50% of the assembled genome) and 30,424 protein-coding genes were identified in the genome. This study provides essential genomic data for further research on the evolution and conservation of . Meanwhile, the high-quality genome assembly also provides insights into the genomic evolution of the genus .

摘要

红鳍方口鲃是一种原产于云南的濒危物种,为澄江特有。由于外来鱼类物种的入侵,该物种的自然种群数量急剧下降。幸运的是,红鳍方口鲃的人工驯化和繁殖已获成功,该物种已成为当地的经济物种。然而,关于红鳍方口鲃的研究仍然很少。在本研究中,组装并注释了红鳍方口鲃的高质量基因组。该基因组使用PacBio平台和Hi-C方法进行测序和组装。基因组大小为1.01 Gb,N50为37.99 Mb。组装的重叠群被锚定到24条染色体上。BUSCO分析表明,基因组组装的基因覆盖完整性为95.6%。基因组中总共鉴定出455.62 Mb的重复序列(占组装基因组的48.50%)和30424个蛋白质编码基因。本研究为红鳍方口鲃的进化和保护进一步研究提供了重要的基因组数据。同时,高质量的基因组组装也为该属的基因组进化提供了见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2daa/11675437/9f348d8c2a75/genes-15-01536-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2daa/11675437/9e14f1e52bad/genes-15-01536-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2daa/11675437/030bc47d1da1/genes-15-01536-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2daa/11675437/0b457d899ac9/genes-15-01536-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2daa/11675437/9f348d8c2a75/genes-15-01536-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2daa/11675437/9e14f1e52bad/genes-15-01536-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2daa/11675437/030bc47d1da1/genes-15-01536-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2daa/11675437/0b457d899ac9/genes-15-01536-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2daa/11675437/9f348d8c2a75/genes-15-01536-g004.jpg

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