da Silva Peterson F F, da Silva Renata R, Sarubbo Leonie A, Guerra Jenyffer M C
Centro de Tecnologia e Geociências, Programa de Pós-Graduação em Engenharia Química, Departamento de Engenharia Química, Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, n. 1235, Cidade Universitária, Recife 50670-901, PE, Brazil.
Rede Nordeste de Biotecnologia (RENORBIO), Universidade Federal Rural de Pernambuco (UFRPE), Rua Dom Manuel de Medeiros, s/n-Dois Irmãos, Recife 52171-900, PE, Brazil.
Foods. 2024 Dec 13;13(24):4029. doi: 10.3390/foods13244029.
Optimizing biosurfactant (BS) production is key for sustainable industrial applications. This study investigated BS synthesis by using licuri oil, a renewable carbon source rich in medium-chain fatty acids. Process optimization was conducted via central composite design (CCD), adjusting concentrations of licuri oil, glucose, NHNO, and yeast extract. The predictive model achieved an R of 0.9451 and adjusted R of 0.8812. Under optimized conditions, lowered water surface tension from 71.04 mN·m to 28.66 mN·m, with a critical micelle concentration (CMC) of 0.8 g·L. The biosurfactant displayed high emulsification indices, exceeding 70% for canola, licuri, and motor oils, suggesting strong potential as an industrial emulsifier. FTIR and NMR analyses confirmed its glycolipid structure. Bioassays showed no toxicity to seeds, ensuring environmental safety, while antimicrobial tests demonstrated efficacy against and , indicating its suitability as a biocidal agent. This work positions BS from licuri oil as a promising alternative for bioremediation, biotechnology, and antimicrobial uses.
优化生物表面活性剂（BS）的生产是可持续工业应用的关键。本研究通过使用富含中链脂肪酸的可再生碳源——licuri油来研究BS的合成。通过中心复合设计（CCD）进行工艺优化，调整licuri油、葡萄糖、NHNO和酵母提取物的浓度。预测模型的R值为0.9451，调整后的R值为0.8812。在优化条件下，水的表面张力从71.04 mN·m降至28.66 mN·m，临界胶束浓度（CMC）为0.8 g·L。该生物表面活性剂具有较高的乳化指数，对油菜籽、licuri油和机油的乳化指数超过70%，表明其作为工业乳化剂具有很强的潜力。傅里叶变换红外光谱（FTIR）和核磁共振（NMR）分析证实了其糖脂结构。生物测定表明对种子无毒，确保了环境安全性，而抗菌测试表明对[具体微生物1]和[具体微生物2]有效，表明其适合作为杀菌剂。这项工作将licuri油产生的BS定位为生物修复、生物技术和抗菌用途的有前途的替代品。
需注意，原文中“NHNO”表述有误，可能影响准确理解，正常应为如“NH₄NO₃”等规范的化学物质名称。
