Wang Zhaohui, Ye Ruihua, Zhang Shidi, Liu Chuanming, Chen Ke, Zhu Kongdi, Wang Pengjie, Wang Fuqing, Huang Jiaqiang
Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, Ministry of Education, China Agricultural University, Beijing 100083, China.
College of Veterinary Medicine, China Agricultural University, Beijing 100083, China.
Foods. 2024 Dec 20;13(24):4138. doi: 10.3390/foods13244138.
Chicken yolk immunoglobulin (IgY) is a natural immunologically active antibody extracted from egg yolk and can be used as a natural dietary supplement for the treatment of inflammation and damage to the intestines. In our study, IgY was embedded in a double emulsion (W/O/W; DE) to explore the therapeutic effect of the embedded IgY on Lipopolysaccharide (LPS)-induced jejunal injury in mice. The results showed that W/O/W-embedded IgY as a dietary supplement (IgY + DE) attenuated LPS-induced damage to mouse small intestinal structures and protected the integrity of the jejunal mucosal barrier. IgY + DE increased the amount of related transcription factors (Math1, Spdef, Elf3, and Klf4) and promoted thrush cell differentiation. IgY + DE ameliorated LPS-induced reduction in mucin quantity and markers. It promoted the expression of Muc1 and Muc2 and increased the mRNA expression levels of Muc1, Muc2, Muc3, Muc4, Muc13, and Agr2 ( < 0.05). IgY + DE increased the expression of several glycosyltransferases involved in mucin glycosylation. IgY + DE also neutralized the LPS attack on the expression of jejunal inflammatory factors IL-1β, IL-6, IL-4, and TNF-α. In conclusion, the IgY-embedded double emulsion can be used as a dietary supplement for immunotherapy to prevent LPS-induced jejunal injury in mice.
鸡卵黄免疫球蛋白（IgY）是一种从蛋黄中提取的天然免疫活性抗体，可作为一种天然膳食补充剂用于治疗肠道炎症和损伤。在我们的研究中，将IgY包埋于复乳（水包油包水；DE）中，以探讨包埋的IgY对脂多糖（LPS）诱导的小鼠空肠损伤的治疗效果。结果表明，作为膳食补充剂的水包油包水包埋IgY（IgY + DE）减轻了LPS诱导的小鼠小肠结构损伤，并保护了空肠黏膜屏障的完整性。IgY + DE增加了相关转录因子（Math1、Spdef、Elf3和Klf4）的数量，并促进了潘氏细胞分化。IgY + DE改善了LPS诱导的黏蛋白数量和标志物的减少。它促进了Muc1和Muc2的表达，并增加了Muc1、Muc2、Muc3、Muc4、Muc13和Agr2的mRNA表达水平（<0.05）。IgY + DE增加了几种参与黏蛋白糖基化的糖基转移酶的表达。IgY + DE还中和了LPS对空肠炎症因子IL-1β、IL-6、IL-4和TNF-α表达的攻击。总之，包埋IgY的复乳可作为一种用于免疫治疗的膳食补充剂，以预防LPS诱导的小鼠空肠损伤。
