Cherneva E
Vet Med Nauki. 1979;16(9):96-102.
Three Escherichia coli strains--302(O111), 311(O78), and 331(O2) as well as streptomycin sensitive and streptomycin resistant mutants obtained from them by mutagenesis were studied. The sensitivity of all strains to streptomycin was tested in dilution series on hard and fluid medium. Growth curves were worked out by the biophotometer of Bonet, Maury and Jonan, adding various streptomycin concentrations to the medium at the very beginning of the experiment and 90 min after that. It was established that streptomycin concentrations above 15 microgram/cm3 added to bacteria sensitive to it at the beginning of their log-phase, shortens its duration by about 40%.
研究了三株大肠杆菌菌株——302(O111)、311(O78)和331(O2),以及通过诱变从它们获得的链霉素敏感和链霉素抗性突变体。在固体和液体培养基上,通过稀释系列试验测试了所有菌株对链霉素的敏感性。使用博内、莫里和乔南的生物光度计绘制生长曲线,在实验开始时以及90分钟后向培养基中添加不同浓度的链霉素。结果表明,在对数期开始时向对链霉素敏感的细菌中添加高于15微克/立方厘米的链霉素浓度,会使其持续时间缩短约40%。