Eisenstein B I, Beachey E H, Ofek I
Scand J Infect Dis Suppl. 1982;33:108-14.
Sublethal concentrations of antibiotics that inhibit protein synthesis were found to diminish the ability of strains of Escherichia coli to bind to human leukocytes and to oral epithelial cells, guinea pig erythrocytes, and mannan-containing yeast cells. In general depressed adherence correlated with diminished production of type 1 fimbriae by the drug-treated bacteria and was affected by the antibiotics in the following order: aminoglycoside (gentamicin, streptomycin, neomycin) greater than spectinomycin greater than tetracycline greater than chloramphenicol. With one notable exception, mutation to resistance to antibiotic-induced growth inhibition resulted in resistance to the sublethal, anti-adherence effects of the same antibiotic. The exceptional strain, VL-2, was the only one out of many streptomycin-resistant strains that was not also resistant to the anti-adherence effects of subinhibitory concentrations of the drug. Compared to control cultures of VL-2, those grown in antibiotic demonstrated decreased amounts of mannose-sensitive hemagglutination (by greater than 99%) and adherence to human epithelial cells (58%) and leukocytes (93%). When treated bacteria were examined by electron microscopy, they were found to be as heavily fimbriate as control bacteria, but their fimbriae were twice as long. Moreover, fimbriae isolated and purified from drug-treated bacteria had no lectin-like properties. Thus, although most antibiotics diminished the adhesive properties only the antibiotics-sensitive bacteria, streptomycin caused one streptomycin-resistant strain of bacteria to produce aberrant (non-adhesive) fimbrial protein.
研究发现，抑制蛋白质合成的亚致死浓度抗生素会降低大肠杆菌菌株与人类白细胞、口腔上皮细胞、豚鼠红细胞以及含甘露聚糖的酵母细胞结合的能力。一般来说，细菌黏附能力降低与经药物处理的细菌1型菌毛产量减少相关，且受抗生素影响的顺序如下：氨基糖苷类（庆大霉素、链霉素、新霉素）＞壮观霉素＞四环素＞氯霉素。除一个显著例外情况外，对抗生素诱导的生长抑制产生抗性的突变会导致对同一抗生素的亚致死抗黏附作用产生抗性。这个例外菌株VL - 2是众多链霉素抗性菌株中唯一对亚抑制浓度药物的抗黏附作用不产生抗性的菌株。与VL - 2的对照培养物相比，在抗生素中生长的培养物表现出甘露糖敏感血凝反应量减少（超过99%）以及对人类上皮细胞（58%）和白细胞（93%）的黏附能力降低。当通过电子显微镜检查经处理的细菌时，发现它们的菌毛与对照细菌一样密集，但菌毛长度是对照细菌的两倍。此外，从经药物处理的细菌中分离和纯化的菌毛没有凝集素样特性。因此，尽管大多数抗生素仅降低了抗生素敏感细菌的黏附特性，但链霉素却使一株链霉素抗性细菌产生了异常（无黏附性）的菌毛蛋白。
