Tsamesidis Ioannis, Papadimitriou-Tsantarliotou Aliki, Christodoulou Athanasios, Amanatidou Dionysia, Avgeros Chrysostomos, Stalika Evangelia, Bousnaki Maria, Michailidou Georgia, Beketova Anastasia, Eleftheriou Phaedra, Bikiaris Dimitrios N, Vizirianakis Ioannis S, Kontonasaki Eleana
Department of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
Laboratory of Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
Biomedicines. 2024 Nov 24;12(12):2674. doi: 10.3390/biomedicines12122674.
, commonly known as absinthe, is a perennial plant with distinctive broad ovate pointed leaves of a silvery-gray color, reaching a height of 1.5 m. The utilization of this herb as a source of natural compounds and as the primary ingredient in the alcoholic beverage absinthe has recently seen a resurgence following a period of prohibition. This study investigates the biological effects of extract on healthy human periodontal ligament stem cells (hPDLSCs) and the human tongue squamous carcinoma cell line (HSC-3). element characterization was performed using High-Performance Liquid Chromatography (HPLC) and the Folin method. Alizarin assays evaluated the osteogenic capacity of human periodontal ligament cells (hPDLSCs) while CCK-8 and MTT determined the cytotoxicity of the extract against HSC-3 and hPDLSCs. High artemisinin levels were detected, revealing a concentration of 89 μM (25 μg/mL). The total phenolic concentration of the extract was 1.07 mM +/- 0.11. The in vitro cytotoxicity assays revealed the biocompatible profile of the extract in hPDLSCs without exhibiting any osteogenic potential. After 24 h of incubation with HSC-3, extract (10 µM) decreased cancer cell viability by 99% and artemisinin by 64%, and increased the expression of Caspase 3 and 9 almost six and two times, respectively. In summary, our preliminary findings suggest that extract exhibits a toxic effect against carcinoma cell lines without affecting healthy human periodontal ligament stem cells.
，俗称苦艾，是一种多年生植物，有着独特的银灰色宽卵形尖叶，高可达1.5米。在经历一段时间的禁令后，这种草药作为天然化合物来源以及作为酒精饮料苦艾酒的主要成分的应用近来再度兴起。本研究调查了[提取物名称]提取物对健康人牙周膜干细胞（hPDLSCs）和人舌鳞状癌细胞系（HSC - 3）的生物学效应。使用高效液相色谱法（HPLC）和福林法进行了[提取物名称]元素表征。茜素测定评估了人牙周膜细胞（hPDLSCs）的成骨能力，而CCK - 8和MTT测定了该提取物对HSC - 3和hPDLSCs的细胞毒性。检测到高青蒿素水平，显示浓度为89μM（25μg/mL）。提取物的总酚浓度为1.07 mM +/- 0.11。体外细胞毒性测定显示[提取物名称]提取物在hPDLSCs中具有生物相容性，且未表现出任何成骨潜力。与HSC - 3孵育24小时后，[提取物名称]提取物（10μM）使癌细胞活力降低99%，青蒿素使其降低64%，并使半胱天冬酶3和9的表达分别增加近6倍和2倍。总之，我们的初步研究结果表明，[提取物名称]提取物对癌细胞系表现出毒性作用，而不影响健康的人牙周膜干细胞。
