Bordean Maria-Evelina, Ungur Rodica Ana, Toc Dan Alexandru, Borda Ileana Monica, Marțiș Georgiana Smaranda, Pop Carmen Rodica, Filip Miuța, Vlassa Mihaela, Nasui Bogdana Adriana, Pop Anamaria, Cinteză Delia, Popa Florina Ligia, Marian Sabina, Szanto Lidia Gizella, Muste Sevastița
Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 64 Calea Floresti, 400509 Cluj-Napoca, Romania.
Centre for Technology Transfer-BioTech, 64 Calea Florești, 400509 Cluj-Napoca, Romania.
Antioxidants (Basel). 2023 Feb 27;12(3):596. doi: 10.3390/antiox12030596.
Taking into account the increasing number of antibiotic-resistant bacteria, actual research focused on plant extracts is vital. The aim of our study was to investigate leaf and stem ethanolic extracts of L. and L. in order to explore their antioxidant and antibacterial activities. Total phenolic content (TPC) was evaluated spectrophotometrically. Antioxidant activity was evaluated by DPPH and ABTS. The antibacterial activity of wormwood extracts was assessed by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in , , , and cultures, and by zone of inhibition in carbapenem-resistant enterobacteriaceae (CRE) and extended-spectrum β-lactamases cultures (ESBL). The L. leaf extract (AnL) exhibited the highest TPC (518.09 mg/mL) and the highest expression of sinapic acid (285.69 ± 0.002 µg/mL). Nevertheless, the highest antioxidant capacity (1360.51 ± 0.04 µM Trolox/g DW by ABTS and 735.77 ± 0.02 µM Trolox/g DW by DPPH) was found in L. leaf from the second year of vegetation (AbL2). AnL extract exhibited the lowest MIC and MBC for all tested bacteria and the maximal zone of inhibition for CRE and ESBL. Our study revealed that AbL2 exhibited the best antioxidant potential, while AnL extract had the strongest antibacterial effect.
考虑到抗生素耐药菌数量的不断增加，针对植物提取物的实际研究至关重要。我们研究的目的是调查[具体植物1]和[具体植物2]的叶和茎乙醇提取物，以探索它们的抗氧化和抗菌活性。通过分光光度法评估总酚含量（TPC）。通过DPPH和ABTS评估抗氧化活性。艾草提取物的抗菌活性通过在[具体培养基1]、[具体培养基2]、[具体培养基3]和[具体培养基4]培养物中的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）以及在耐碳青霉烯类肠杆菌科细菌（CRE）和产超广谱β-内酰胺酶培养物（ESBL）中的抑菌圈来评估。[具体植物1]叶提取物（AnL）表现出最高的TPC（518.09毫克/毫升）和芥子酸的最高表达量（285.69±0.002微克/毫升）。然而，在第二年生长的[具体植物2]叶（AbL2）中发现了最高的抗氧化能力（通过ABTS为1360.51±0.04微摩尔Trolox/克干重，通过DPPH为735.77±0.02微摩尔Trolox/克干重）。AnL提取物对所有测试细菌表现出最低的MIC和MBC以及对CRE和ESBL的最大抑菌圈。我们的研究表明，AbL2表现出最佳的抗氧化潜力，而AnL提取物具有最强的抗菌效果。
