Saito Y, Sakamoto H, MacLusky N J, Naftolin F
Am J Obstet Gynecol. 1985 Mar 15;151(6):805-12. doi: 10.1016/0002-9378(85)90525-3.
Myometrial gap junctions, levels of cytosol and nuclear estradiol and progesterone receptors, and serum estradiol and progesterone levels were determined simultaneously in pregnant and postpartum rats. Between days 15 to 20 after conception, levels of progesterone nuclear receptors decreased (776.8 +/- 88.5 versus 241.1 +/- 56.5 fmol/mg of DNA, p less than 0.05). The mean serum progesterone also fell by about 50% during this period, but variation in individual levels between days 15 to 20 did not allow for this group to achieve statistical significance until day 21. As the progesterone nuclear receptors decreased, estradiol remained stable, but estradiol nuclear receptors increased (day 21 versus 22: 1710.6 +/- 61.1 versus 3254.8 +/- 203.8 fmol/mg of DNA, p less than 0.05), preceding the increase in gap junctions observed at parturition (day 21 versus 22: 2.0 +/- 0.2 versus 8.0 +/- 3.2 [per 1000 micron plasma membrane], p less than 0.05). Gap junctions fell to prepartum levels by 12 hours, when progesterone nuclear receptors were markedly increasing (6 versus 12 hours post partum: 637.7 +/- 324.8 versus 1509.6 +/- 283.2 fmol/mg of DNA, p less than 0.05). Relationships between gap junctions and cellular estradiol and progesterone nuclear receptors are clearer than could be forecast by circulating hormone measurements alone, and may offer a cellular basis for the role of progesterone in controlling labor.
在怀孕和产后大鼠中同时测定子宫肌层缝隙连接、胞质和核雌二醇及孕酮受体水平以及血清雌二醇和孕酮水平。在受孕后第15至20天之间，孕酮核受体水平下降（776.8±88.5对241.1±56.5 fmol/mg DNA，p<0.05）。在此期间，血清孕酮平均水平也下降了约50%，但在第15至20天之间个体水平的变化使得该组直到第21天才达到统计学显著性。随着孕酮核受体减少，雌二醇保持稳定，但雌二醇核受体增加（第21天与第22天：1710.6±61.1对3254.8±203.8 fmol/mg DNA，p<0.05），早于分娩时观察到的缝隙连接增加（第21天与第22天：2.0±0.2对8.0±3.2[每1000微米质膜]，p<0.05）。缝隙连接在产后12小时降至产前水平，此时孕酮核受体显著增加（产后6小时与12小时：637.7±324.8对1509.6±283.2 fmol/mg DNA，p<0.05）。缝隙连接与细胞雌二醇和孕酮核受体之间的关系比仅通过循环激素测量所能预测的更为清晰，并且可能为孕酮在控制分娩中的作用提供细胞基础。
