Sato Kazuomi, Hiraga Yosuke, Yamaguchi Yuji, Sakaki Setsuko, Takenaka Hiroyuki
Graduate School of Agriculture, Tamagawa University, 6-1-1 Tamagawa-gakuen, Tokyo 194-8610, Japan.
MAC Gifu Research Institute, MicroAlgae Corporation, 4-15 Akebono-cho, Gifu 500-8148, Japan.
Life (Basel). 2024 Nov 25;14(12):1542. doi: 10.3390/life14121542.
This study investigated the multifaceted benefits of water extract across various cell lines, including murine B16F1 melanoma cells, human keratinocyte HaCaT cells, and human follicle dermal papilla cells (HFDPCs), to assess its potential in skin health improvement. Initially, the antioxidant capacity of the extract was evaluated using the ABTS assay, revealing significant radical scavenging activity, indicating strong antioxidative properties. Subsequently, extract showed notable inhibition of α-MSH-enhanced melanin production in B16F1 cells without cell toxicity by suppressing tyrosinase expression. Furthermore, the extract significantly promoted cell migration and enhanced wound healing in HaCaT cells, accompanied by an upregulation of VEGF and MMP mRNA levels, which are crucial for the wound healing process. In addition, we investigated the effect of extract on hair growth-related genes in HFDPCs. Despite a slight reduction in VEGF mRNA levels, an increase in CTGF and HGF1 mRNA levels was observed, alongside a significant down-regulation of TGFβ1, highlighting the extract's potential to promote hair growth and exhibit antiandrogenic effects. Collectively, these findings demonstrated the therapeutic potential of extract in treating hyperpigmentation, enhancing wound healing, and promoting hair growth, making it a promising candidate for future dermatological applications.
本研究调查了水提取物对多种细胞系的多方面益处，包括小鼠B16F1黑色素瘤细胞、人角质形成细胞HaCaT细胞和人毛囊真皮乳头细胞（HFDPCs），以评估其在改善皮肤健康方面的潜力。最初，使用ABTS法评估提取物的抗氧化能力，结果显示出显著的自由基清除活性，表明其具有强大的抗氧化性能。随后，提取物通过抑制酪氨酸酶表达，在不产生细胞毒性的情况下，对B16F1细胞中α-MSH增强的黑色素生成表现出显著抑制作用。此外，提取物显著促进HaCaT细胞的迁移并增强伤口愈合，同时伴随着VEGF和MMP mRNA水平的上调，这对伤口愈合过程至关重要。此外，我们研究了提取物对HFDPCs中毛发生长相关基因的影响。尽管VEGF mRNA水平略有降低，但观察到CTGF和HGF1 mRNA水平升高，同时TGFβ1显著下调，突出了提取物促进毛发生长和展现抗雄激素作用的潜力。总体而言，这些发现证明了提取物在治疗色素沉着、促进伤口愈合和促进毛发生长方面的治疗潜力，使其成为未来皮肤科应用的有希望的候选物。
