Aslan Ali, Seçme Mücahit
Department of Physiology, Faculty of Medicine, Ordu University, Ordu 52200, Turkey.
Department of Medical Biology, Faculty of Medicine, Ordu University, Ordu 52200, Turkey.
Medicina (Kaunas). 2024 Dec 23;60(12):2110. doi: 10.3390/medicina60122110.
Neuroblastoma is the most common extracranial solid tumor in children, often presenting challenges in treatment due to its clinical and genetic heterogeneity. This study investigated the anticancer potential of root extract on the human neuroblastoma cell line (SH-SY5Y). Using XTT assays, ELISA-based oxidative stress markers, and RT-PCR analysis of apoptotic genes, the study explored the extract's effects on cell proliferation, oxidative stress, and apoptosis. For the cell culture, SH-SY5Y human neuroblastoma cells were thawed, cultured, and maintained under appropriate conditions for experiments. The dose- and time-dependent activity of Pelorgonium sidoides extract on SH-SY5Y neuroblastoma cells was investigated by XTT assay. The change in the oxidative stress marker 8-Hydroxy-2'-deoxyguanosine (8-OhDG) level was determined by ELISA for the doses applied to the control group root extract at a concentration of 25 μg/mL. Total antioxidant status (TAS) and total oxidant status (TOS) were measured from the cells in the study group with the help of a commercial kit. The oxidative stress index (OSI) was calculated by dividing the TAS by the TOS and multiplying by 100. In order to evaluate the expression levels of apoptosis-related Bax, Bcl-2, Caspase-3, Caspase-8, and Caspase-9 genes at the mRNA level in control and dose group cells, RNA isolation was performed from the SH-SY5Y control and dose group cells (IC50 value). It is observed that the substance inhibits proliferation in cells at 24 h ( < 0.05). As the dose increases, cell proliferation decreases ( < 0.05). The IC50 value was calculated to be 113.83 μg/mL at 24 h. The concentration of 8-OhDG increased in neuroblastoma cells as a result of extract treatment ( < 0.05). TOS levels increased in neuroblastoma cells treated with extract ( < 0.01). OSI levels increased in cells treated with extract ( < 0.001). BAX and Caspase-8 expression increased are statistically significant in the dose group ( < 0.05). extract induces apoptosis in neuroblastoma cells through oxidative stress and mitochondrial- and death receptor-mediated pathways. This study highlights the potential of as a complementary therapeutic agent for neuroblastoma, warranting further in vivo and clinical investigations to assess its safety and efficacy.
神经母细胞瘤是儿童最常见的颅外实体瘤，由于其临床和基因异质性，在治疗中常常面临挑战。本研究调查了[植物名称]根提取物对人神经母细胞瘤细胞系（SH-SY5Y）的抗癌潜力。该研究使用XTT检测、基于酶联免疫吸附测定（ELISA）的氧化应激标志物以及凋亡基因的逆转录聚合酶链反应（RT-PCR）分析，探讨了提取物对细胞增殖、氧化应激和凋亡的影响。对于细胞培养，将SH-SY5Y人神经母细胞瘤细胞解冻、培养并在适当条件下维持用于实验。通过XTT检测研究了[植物名称]提取物对SH-SY5Y神经母细胞瘤细胞的剂量和时间依赖性活性。通过ELISA测定应用于对照组根提取物浓度为25μg/mL时氧化应激标志物8-羟基-2'-脱氧鸟苷（8-OhDG）水平的变化。借助商业试剂盒从研究组细胞中测量总抗氧化状态（TAS）和总氧化状态（TOS）。通过将TAS除以TOS并乘以100来计算氧化应激指数（OSI）。为了评估对照和剂量组细胞中凋亡相关的Bax、Bcl-2、Caspase-3、Caspase-8和Caspase-9基因在mRNA水平的表达，从SH-SY5Y对照和剂量组细胞（IC50值）中进行RNA分离。观察到该物质在24小时时抑制细胞增殖（P<0.05）。随着剂量增加，细胞增殖降低（P<0.05）。计算得出24小时时IC50值为113.83μg/mL。提取物处理后神经母细胞瘤细胞中8-OhDG的浓度增加（P<0.05）。提取物处理的神经母细胞瘤细胞中TOS水平增加（P<0.01）。提取物处理的细胞中OSI水平增加（P<0.001）。剂量组中BAX和Caspase-8表达增加具有统计学意义（P<0.05）。提取物通过氧化应激以及线粒体和死亡受体介导的途径诱导神经母细胞瘤细胞凋亡。本研究突出了[提取物名称]作为神经母细胞瘤辅助治疗剂的潜力，需要进一步进行体内和临床研究以评估其安全性和有效性。
