Shnaider Polina V, Malyants Irina K, Ivanova Olga M, Gordeeva Veronika S, Svirina Ekaterina A, Zakharzhevskaya Natalya B, Shagaleeva Olga Y, Selezneva Oksana V, Bogomazova Alexandra N, Lukina Maria M, Aleshikova Olga I, Babaeva Nataliya A, Slonov Andrey V, Shender Victoria O
Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia.
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of the Federal Medical and Biological Agency, 119435 Moscow, Russia.
Int J Mol Sci. 2024 Dec 10;25(24):13236. doi: 10.3390/ijms252413236.
High-grade serous ovarian carcinoma (HGSOC) remains the most common and deadly form of ovarian cancer. However, available cell lines usually fail to appropriately represent its complex molecular and histological features. To overcome this drawback, we established OVAR79, a new cell line derived from the ascitic fluid of a patient with a diagnosis of HGSOC, which adds a unique set of properties to the study of ovarian cancer. In contrast to the common models, OVAR79 expresses without the common hotspot mutations and harbors the rare combination of mutations in both PIK3CA and PTEN genes, together with high-grade chromosomal instability with multiple gains and losses. These features, together with the high proliferation rate, ease of cultivation, and exceptional transfection efficiency of OVAR79, make it a readily available and versatile tool for various studies in the laboratory. We extensively characterized its growth, migration, and sensitivity to platinum- and taxane-based treatments in comparison with the commonly used SKOV3 and OVCAR3 ovarian cell lines. In summary, OVAR79 is an excellent addition for basic and translational ovarian cancer research and offers new insights into the biology of HGSOC.
高级别浆液性卵巢癌（HGSOC）仍然是最常见且致命的卵巢癌形式。然而，现有的细胞系通常无法恰当地体现其复杂的分子和组织学特征。为克服这一缺陷，我们建立了OVAR79，这是一种源自一名被诊断为HGSOC患者腹水的新细胞系，它为卵巢癌研究增添了一组独特的特性。与常见模型不同，OVAR79不表达常见的热点突变，同时携带PIK3CA和PTEN基因中罕见的突变组合，以及伴有多次增减的高级别染色体不稳定性。这些特征，再加上OVAR79的高增殖率、易于培养和出色的转染效率，使其成为实验室各种研究中随时可用且多功能的工具。与常用的SKOV3和OVCAR3卵巢癌细胞系相比，我们广泛地对其生长、迁移以及对铂类和紫杉烷类治疗的敏感性进行了表征。总之，OVAR79是基础和转化性卵巢癌研究的优秀补充，并为HGSOC的生物学研究提供了新的见解。
