Ueda Shusuke, Ichiseki Toru, Shimasaki Miyako, Soma Daisuke, Sakurai Masaru, Kaneuji Ayumi, Kawahara Norio
Department of Orthopaedic Surgery, Kanazawa Medical University, Daigaku 1-1, Uchinada-machi, Kahoku-gun 920-0293, Japan.
Division of Translational Research, Department of Life Science, Medical Research Institute, Kanazawa Medical University, Daigaku 1-1, Uchinada-machi, Kahoku-gun 920-0293, Japan.
Int J Mol Sci. 2024 Dec 12;25(24):13358. doi: 10.3390/ijms252413358.
Tendinopathy is an aging-related disease, often caused by micro-scarring and degeneration due to overuse or trauma. Ascorbic acid (vitamin C) supplementation is reported to be a useful treatment for tendinopathy recovery. We compared the inhibitory effects of various ascorbic acid doses on tendon cell damage. HO was added to human-derived tendon cells in vitro (Group HO, control), followed by incubation with 150 µM or 30 mM of ascorbic acid (Group C, Group HC). The oxidative injury degree was evaluated by determining reactive oxygen species levels. The cytoskeletal structure was examined via fluorescence immunostaining of actin filaments. Quantitative polymerase chain reaction (qPCR) was performed to analyze the expressions of mitochondria transcription factor A, adenosine triphosphate 5A, type I collagen, and p16. Cell death was reduced, and oxidative stress was inhibited in C and HC groups. The cytoskeleton was maintained in the HC group but not in the C group. qPCR analysis revealed that p16 expression was inhibited in both the C and HC groups compared to the HO group; other markers had increased expression. The progression of cell death and cytoskeletal disruption was inhibited by the administration of high-dose vitamin C. Hence, high-dose vitamin C is a potential treatment for tendon cell degeneration.
肌腱病是一种与衰老相关的疾病，通常由过度使用或创伤导致的微疤痕形成和退变引起。据报道，补充抗坏血酸（维生素C）是肌腱病恢复的一种有效治疗方法。我们比较了不同剂量抗坏血酸对肌腱细胞损伤的抑制作用。在体外将血红素加至人源肌腱细胞中（血红素组，对照组），随后分别用150µM或30mM抗坏血酸孵育（C组、高剂量抗坏血酸组）。通过测定活性氧水平评估氧化损伤程度。通过肌动蛋白丝的荧光免疫染色检查细胞骨架结构。进行定量聚合酶链反应（qPCR）分析线粒体转录因子A、三磷酸腺苷5A、I型胶原蛋白和p16的表达。C组和高剂量抗坏血酸组细胞死亡减少，氧化应激受到抑制。高剂量抗坏血酸组细胞骨架得以维持，而C组则未维持。qPCR分析显示，与血红素组相比，C组和高剂量抗坏血酸组p16表达均受到抑制；其他标志物表达增加。高剂量维生素C给药可抑制细胞死亡和细胞骨架破坏的进展。因此，高剂量维生素C是治疗肌腱细胞退变的一种潜在方法。
