Oczkowicz Jarosław, Piasna-Słupecka Ewelina, Drozdowska Mariola, Koronowicz Aneta, Kopeć Aneta
Department of Human Nutrition and Dietetics, University of Agriculture, Balicka 122, 30-149 Kraków, Poland.
Int J Mol Sci. 2024 Dec 14;25(24):13429. doi: 10.3390/ijms252413429.
Consuming food containing ingredients with a documented impact on lipid metabolism can help fight overweight and obesity. The simplest way to reduce the level of fatty acids is to block their synthesis or increase the rate of their degradation. This study aimed to determine the effect of resveratrol, , conjugated linoleic acid (CLA), , CLA, and various variants of their combinations on de novo fatty acid biosynthesis in 3T3-L1 adipocytes. The influence of the above-mentioned bioactive substances on cells grown under standard conditions and after induction of oxidative stress was measured. The effect of the tested compounds on the expression of selected genes related to the de novo fatty acid biosynthesis process (Fasn, Acc1, Acly, Prkaa1, Prkaa2, Prkaca, Srebp1) was evaluated. As part of the conducted experiments, how the level of the corresponding mRNA translates into the content of selected proteins (acetyl-CoA carboxylase 1 (ACC) and fatty acid synthase (FASN) was studied. It was found that the inhibition of fatty acid biosynthesis processes was stronger in the case of the combination of the tested CLA isomers (, CLA, , CLA) with resveratrol than in cases of their individual action.
食用含有对脂质代谢有明确影响成分的食物有助于对抗超重和肥胖。降低脂肪酸水平最简单的方法是阻断其合成或提高其降解速率。本研究旨在确定白藜芦醇、共轭亚油酸（CLA）、CLA及其各种组合变体对3T3-L1脂肪细胞中从头脂肪酸生物合成的影响。测定了上述生物活性物质对在标准条件下生长的细胞以及氧化应激诱导后的细胞的影响。评估了受试化合物对与从头脂肪酸生物合成过程相关的选定基因（Fasn、Acc1、Acly、Prkaa1、Prkaa2、Prkaca、Srebp1）表达的影响。作为所进行实验的一部分，研究了相应mRNA水平如何转化为选定蛋白质（乙酰辅酶A羧化酶1（ACC）和脂肪酸合酶（FASN））的含量。结果发现，受试CLA异构体（CLA、CLA）与白藜芦醇组合对脂肪酸生物合成过程的抑制作用比它们单独作用时更强。
