Geng Xu, Wei Yan, Li Yuanxin, Zhao Siqi, Li Zhengqiang, Li Heng, Li Chen
Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, China.
State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
Int J Mol Sci. 2024 Dec 18;25(24):13541. doi: 10.3390/ijms252413541.
Microbial attack, particularly fungal degradation of cellulose, is a leading cause of paper damage. To address fungal spores and the rising concern of microbial drug resistance, a nano-Germanium dioxide (GeO)/cetyltrimethylammonium bromide (CTAB) complex (nano-GeO/CTAB complex) with potent antibacterial properties was synthesized. Its inhibitory effects were evaluated against bacteria, including Gram-positive and Gram-negative , as well as fungi isolated from paper ( spp., spp., and ). The nano-GeO/CTAB complex exhibited significant ( < 0.05) inhibitory effects against and . Moreover, a 60 min treatment with 1 mg/mL of the complex significantly inhibited the growth of all tested fungi and reduced their biomass after five days of culture, while 4 mg/mL completely deactivated spores. Filter paper pre-treated with the nano-GeO/CTAB complex showed complete resistance to microbial attack, exhibiting no fungal growth and a clear inhibition zone devoid of bacterial growth. In contrast, untreated controls displayed fungal coverage exceeding 95% within five days. These findings highlight the nano-GeO/CTAB complex as a promising antimicrobial agent for protecting paper materials from microbial degradation.
微生物侵袭，尤其是纤维素的真菌降解，是纸张损坏的主要原因。为了解决真菌孢子问题以及日益受到关注的微生物耐药性问题，合成了具有强效抗菌性能的纳米二氧化锗（GeO）/十六烷基三甲基溴化铵（CTAB）复合物（纳米GeO/CTAB复合物）。评估了其对细菌（包括革兰氏阳性菌和革兰氏阴性菌）以及从纸张中分离出的真菌（曲霉属、青霉属和木霉属）的抑制作用。纳米GeO/CTAB复合物对金黄色葡萄球菌和大肠杆菌表现出显著（P < 0.05）的抑制作用。此外，用1 mg/mL的该复合物处理60分钟可显著抑制所有受试真菌的生长，并在培养五天后降低其生物量，而4 mg/mL可使孢子完全失活。用纳米GeO/CTAB复合物预处理的滤纸对微生物侵袭表现出完全抗性，没有真菌生长，且有一个无细菌生长的明显抑菌圈。相比之下，未处理的对照在五天内真菌覆盖率超过95%。这些发现突出了纳米GeO/CTAB复合物作为一种有前景的抗菌剂，可保护纸张材料免受微生物降解。
