Chang Zhi-Min, Wang Zheng, Lu Meng-Meng, Shao Dan, Yue Juan, Yang Dian, Li Ming-Qiang, Dong Wen-Fei
CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Colloids Surf B Biointerfaces. 2017 Sep 1;157:199-206. doi: 10.1016/j.colsurfb.2017.05.079. Epub 2017 Jun 4.
In this study, we exploited a one-pot method to fabricate cetyltrimethylammonium bromide (CTAB)-loaded Janus silver mesoporous silica nanoparticles (Janus Ag-MSNs@CTAB). These bullet-like nanoparticles had a silver head (80nm in diameter) attached to a mesoporous silica stick (200-300nm in length). The CTAB-loaded nanobullets exhibited a marked affinity for the bacterial cell surface and the simultaneously sustained release behavior of CTAB and silver ions. The minimum inhibitory concentrations (MIC) of Janus Ag-MSNs@CTAB were determined to be 10μg/mL and 20μg/mL for E. coli and S. Aureus, respectively. Importantly, Janus Ag-MSNs@CTAB provided a single-particle nanoplatform with a synergistic effect against both Gram-positive and Gram-negative bacteria. A thorough investigation indicated that CTAB induces a dramatic loss of bacterial membrane integrity, which facilitated the internalization of silver. This report described an efficient and convenient method of synthesizing Janus silver mesoporous silica nanoparticles, and these nanobullets show promising potential in biomedical applications.
在本研究中，我们采用一锅法制备了负载十六烷基三甲基溴化铵（CTAB）的Janus银介孔二氧化硅纳米粒子（Janus Ag-MSNs@CTAB）。这些子弹状纳米粒子有一个银质头部（直径80nm）连接在一根介孔二氧化硅棒（长度200 - 300nm）上。负载CTAB的纳米子弹对细菌细胞表面表现出显著的亲和力，以及CTAB和银离子的同时缓释行为。Janus Ag-MSNs@CTAB对大肠杆菌和金黄色葡萄球菌的最低抑菌浓度（MIC）分别测定为10μg/mL和20μg/mL。重要的是，Janus Ag-MSNs@CTAB提供了一个对革兰氏阳性菌和革兰氏阴性菌均具有协同作用的单粒子纳米平台。深入研究表明，CTAB会导致细菌膜完整性急剧丧失，这有利于银的内化。本报告描述了一种高效便捷的合成Janus银介孔二氧化硅纳米粒子的方法，并且这些纳米子弹在生物医学应用中显示出有前景的潜力。
