Kim Eun-A, Kang Nalae, Heo Jun-Ho, Park Areumi, Heo Seong-Yeong, Kim Hyun-Soo, Heo Soo-Jin
Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of Korea.
Department of Seafood Science and Technology, The Institute of Marine Industry, Gyeongsang National University, Tongyeong 53064, Republic of Korea.
Int J Mol Sci. 2024 Dec 21;25(24):13694. doi: 10.3390/ijms252413694.
and its major compound dieckol, both natural marine products, possess antioxidant, anti-inflammatory, and metabolic-regulating effects. Zika virus (ZIKV), an arbovirus from the family, is transmitted by mosquitoes and causes serious illnesses in humans. This study aimed to evaluate the anti-ZIKV potential of and dieckol. The antiviral activity of extract (ECE), prepared with 80% ethanol, was assessed in ZIKV-infected Vero E6 cells through MTT assay, plaque assay, and quantitative polymerase chain reaction (qPCR), demonstrating no cytotoxicity and a significant reduction in viral titers and ZIKV mRNA levels. In addition, ECE decreased the expression of tumor necrosis factor-α and interferon-induced protein with tetratricopeptide repeats in the ZIKV-infected cells. Dieckol, the primary active compound in ECE, exhibited potent anti-ZIKV activity, with a half maximal inhibitory concentration (IC), value of 4.8 µM. In silico molecular docking analysis revealed that dieckol forms stable complexes with key ZIKV proteins, including the envelope, NS2B/NS3, and RNA-dependent RNA polymerase (RdRp) protein, exhibiting high binding energies of -438.09 kcal/mol, -1040.51 kcal/mol, and -1043.40 kcal/mol, respectively. Overall, our findings suggest that ECE and dieckol are promising candidates for the development of anti-ZIKV agents.
及其主要化合物二eckol均为天然海洋产物，具有抗氧化、抗炎和代谢调节作用。寨卡病毒（ZIKV）是一种来自该家族的虫媒病毒，通过蚊子传播，可导致人类患上严重疾病。本研究旨在评估[提取物名称]和二eckol的抗寨卡病毒潜力。用80%乙醇制备的[提取物名称]提取物（ECE）在寨卡病毒感染的Vero E6细胞中通过MTT法、空斑试验和定量聚合酶链反应（qPCR）评估其抗病毒活性，结果表明其无细胞毒性，且病毒滴度和寨卡病毒mRNA水平显著降低。此外，ECE降低了寨卡病毒感染细胞中肿瘤坏死因子-α和含四肽重复序列的干扰素诱导蛋白的表达。ECE中的主要活性化合物二eckol表现出强大的抗寨卡病毒活性，半数最大抑制浓度（IC₅₀）值为4.8 μM。计算机模拟分子对接分析表明，二eckol与寨卡病毒关键蛋白形成稳定复合物，包括包膜蛋白、NS2B/NS3蛋白和RNA依赖性RNA聚合酶（RdRp）蛋白，其结合能分别为-438.09 kcal/mol、-1040.51 kcal/mol和-1043.40 kcal/mol。总体而言，我们的研究结果表明，ECE和二eckol有望成为开发抗寨卡病毒药物的候选物。
