Liu Jisheng, Chen Weijian, Chen Sihua, Li Shuqiang, Swevers Luc
School of Life Sciences, Guangzhou University, Guangzhou 510006, China.
Institute of Biosciences and Applications, National Centre for Scientific Research Demokritos, 15431 Athens, Greece.
Insects. 2024 Dec 19;15(12):1005. doi: 10.3390/insects15121005.
Toll receptors play important roles in the development and innate immunity of insects. Previously, we reported the immunological function of in silkworm, , larvae. In this study, we focused on the role of as a regulator in the Toll signaling pathway. The expressions of most signaling genes in the Toll pathway, as well as immune effectors, were reduced after the RNAi of . Coincidentally, hemolymph from -silenced larvae exhibited decreased antibacterial activity in the growth of , demonstrated either by growth curve or inhibitory zone experiments. The oral administration of heat-inactivated and following the RNAi of up-regulated the expression of most signaling genes in the Toll pathway and downstream immune effectors. The above results indicate that is positively involved in the Toll signaling pathway. As a positive regulator, is shown to be activated preferentially against and, in turn, positively modulates the humoral immune response in antibacterial activity.
Toll受体在昆虫的发育和先天免疫中发挥着重要作用。此前，我们报道了家蚕幼虫中[具体基因名称未给出]的免疫功能。在本研究中，我们聚焦于[具体基因名称未给出]作为Toll信号通路中一种调节因子的作用。[具体基因名称未给出]的RNA干扰后，Toll通路中大多数信号基因以及免疫效应分子的表达均降低。巧合的是，通过生长曲线或抑菌圈实验证明，来自[具体基因名称未给出]沉默幼虫的血淋巴在[细菌名称未给出]生长过程中表现出降低的抗菌活性。在[具体基因名称未给出]的RNA干扰后口服热灭活的[细菌名称未给出]和[细菌名称未给出]上调了Toll通路中大多数信号基因和下游免疫效应分子的表达。上述结果表明[具体基因名称未给出]正向参与Toll信号通路。作为一种正向调节因子，[具体基因名称未给出]被证明优先针对[细菌名称未给出]被激活，进而正向调节抗菌活性中的体液免疫反应。
