Romeo Maria Anele, Specchiarello Eliana, Mija Cosmina, Zulian Verdiana, Francalancia Massimo, Maggi Fabrizio, Garbuglia Anna Rosa, Lapa Daniele
Laboratory of Virology, National Institute for Infectious Disease "Lazzaro Spallanzani"-IRCCS, 00149 Rome, Italy.
Microbiology and Virology Unit, San Gallicano Dermatological Institute, IRCCS, Istituti Fisioterapici Ospedalieri (IFO), 00144 Rome, Italy.
Pathogens. 2024 Dec 10;13(12):1089. doi: 10.3390/pathogens13121089.
Rift Valley Fever virus (RVFV) is a mosquito-borne virus with high pathogenic potential in ruminants and humans. Due to its high potential for spreading, it is considered a priority pathogen, and it is included in the Bluepoint list of the World Health Organization (WHO). Given the high pathogenic potential of the virus, it is crucial to develop a rapid heat-mediated inactivation protocol to create a safer working environment, particularly in medical facilities that lack a biosafety level 3 laboratory required for direct handling of RVFV. Our results reveal the broad tissue tropism of RVFV, showing the virus's capacity for replication in various cell lines. In terms of the thermal stability of RVFV, our findings showed that a 70 °C heat treatment did not fully inactivate the virus within 15 min. However, when exposed to 80 °C and 95 °C, the virus was completely inactivated after 15 min and 5 min, respectively. Additionally, our results indicated that heat-treatment only slightly decreased the integrity of the RVFV genome whether there is a high or low number of viral RNA copies. Overall, the study established a straightforward protocol for heat inactivation that may be beneficial in handling clinical and research samples of RVFV.
裂谷热病毒（RVFV）是一种通过蚊子传播的病毒，对反刍动物和人类具有很高的致病潜力。由于其传播潜力高，它被视为优先病原体，并被列入世界卫生组织（WHO）的蓝点清单。鉴于该病毒的高致病潜力，制定一种快速的热介导灭活方案以创造更安全的工作环境至关重要，特别是在缺乏直接处理RVFV所需的生物安全3级实验室的医疗设施中。我们的结果揭示了RVFV广泛的组织嗜性，表明该病毒在各种细胞系中的复制能力。就RVFV的热稳定性而言，我们的研究结果表明，70°C热处理在15分钟内并未完全灭活病毒。然而，当暴露于80°C和95°C时，病毒分别在15分钟和5分钟后被完全灭活。此外，我们的结果表明，无论病毒RNA拷贝数是高还是低，热处理只会略微降低RVFV基因组的完整性。总体而言，该研究建立了一种简单的热灭活方案，这可能有助于处理RVFV的临床和研究样本。
