Ulas Mevlut, Hussey Seamus, Broderick Annemarie, Fitzpatrick Emer, Dunne Cara, Cooper Sarah, Dominik Anna, Bourke Billy
Department of Paediatrics, University College Dublin, CHI-Crumlin, D12 N512 Dublin, Ireland.
National Children's Research Center, CHI-Crumlin, D12 N512 Dublin, Ireland.
Pathogens. 2024 Dec 13;13(12):1102. doi: 10.3390/pathogens13121102.
Metaproteomic analysis of microbiome post-translation modifications (PTMm) is challenging, and little is known about the effects of inflammation on the bacterial PTM landscape in IBD. Here, we adapted and optimised fluorescence in situ hybridisation-flow cytometry (FISH-FC) to study microbiome-wide tyrosine phosphorylation (p-Tyr) in children with and without inflammatory bowel disease (IBD). Microbial p-Tyr signal was significantly higher in children with IBD, compared to those without. , , and tended to be more abundant in IBD than in non-IBD control children but there were only minor differences in p-Tyr among these bacterial communities in those with and without IBD. p-Tyr was significantly lower in non-IBD children older than 9 yrs compared with those less than 9 yrs, and the effect was seen in all four bacterial subgroups studied. The opposite trend was seen in patients with IBD. p-Tyr overall is higher in children with IBD but the effects of inflammation on p-Tyr vary according to the bacterial community. The overall microbiome p-Tyr signal changes with age in healthy children. FISH-FC can be used to study the microbiome-wide PTM landscape.
微生物组翻译后修饰（PTMm）的宏蛋白质组学分析具有挑战性，关于炎症对炎症性肠病（IBD）中细菌PTM格局的影响知之甚少。在这里，我们对荧光原位杂交-流式细胞术（FISH-FC）进行了调整和优化，以研究患有和未患有炎症性肠病（IBD）的儿童中全微生物组酪氨酸磷酸化（p-Tyr）情况。与未患IBD的儿童相比，患IBD的儿童中微生物p-Tyr信号显著更高。 、 、 和 在IBD中往往比在非IBD对照儿童中更为丰富，但在患有和未患有IBD的儿童中，这些细菌群落之间的p-Tyr只有微小差异。9岁以上的非IBD儿童的p-Tyr明显低于9岁以下的儿童，并且在所有四个研究的细菌亚组中都观察到了这种效应。在IBD患者中观察到相反的趋势。总体而言，IBD儿童的p-Tyr较高，但炎症对p-Tyr的影响因细菌群落而异。健康儿童中全微生物组p-Tyr信号随年龄变化。FISH-FC可用于研究全微生物组PTM格局。
