高分辨率微生物群流式细胞术揭示了粪便细菌组成中与结肠炎相关的动态变化。
High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition.
作者信息
Zimmermann Jakob, Hübschmann Thomas, Schattenberg Florian, Schumann Joachim, Durek Pawel, Riedel René, Friedrich Marie, Glauben Rainer, Siegmund Britta, Radbruch Andreas, Müller Susann, Chang Hyun-Dong
机构信息
German Rheumatism Research Center Berlin (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
Department for Environmental Microbiology, Helmholtz Center for Environmental Research (UFZ), Leipzig, Germany.
出版信息
Eur J Immunol. 2016 May;46(5):1300-3. doi: 10.1002/eji.201646297.
Using high-resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive tool to interrogate the microbiota on the single-cell level.
通过对细菌形态（前向散射）和DNA含量（DAPI染色）进行高分辨率流式细胞术检测，我们发现在小鼠结肠炎期间粪便微生物群组成存在显著差异，这一结果通过16S rDNA测序得到了验证。这种创新方法提供了一种快速且经济的工具，可在单细胞水平上研究微生物群。
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