基于全基因组测序对萨格勒布大学医院中心感染病例的特征分析

Whole-Genome Sequencing-Based Characterization of Infection Cases at the University Hospital Centre Zagreb.

作者信息

Siroglavic Marko, Higgins Paul G, Kanizaj Lucija, Ferencak Ivana, Juric Dragan, Augustin Goran, Budimir Ana

机构信息

Department of Clinical Microbiology, Infection Prevention and Control, University Hospital Centre Zagreb, Kispaticeva st. 12, 10000 Zagreb, Croatia.

Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Goldenfelsstraße 19-21, 50935 Cologne, Germany.

出版信息

Microorganisms. 2024 Nov 27;12(12):2434. doi: 10.3390/microorganisms12122434.

DOI:10.3390/microorganisms12122434

PMID:39770637

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11676685/

Abstract

We investigated the intra-hospital distribution of strains by whole-genome sequencing (WGS) of isolates collected in 2022 at the University Hospital Centre (UHC) Zagreb. In total, 103 patients with first-episode CDI in 2022 at UHC Zagreb were included, based on the screening stool antigen test for GDH (RidaQuick CD GDH; R-Biopharm AG, Germany), confirmed by Eazyplex assays (Eazyplex CD assay; AmplexDiagnostics GmbH, Germany) specific for A, B, and binary toxins. Demographic and clinical data were retrospectively analyzed from electronic medical records. All samples were subjected to WGS analysis. Genetic clusters were formed from isolates with no more than six allelic differences according to core genome MLST. We identified six clusters containing 2-59 isolates with 15 singletons and 30 instances of possible intra-hospital transmission, mostly in the COVID-19 ward. WGS analysis proved useful in identifying clusters of isolates connecting various patient wards with possible transmission routes in the hospital setting. It could be used to support local and national surveillance of CDI infections and their transmission pathways.

摘要

我们通过对2022年在萨格勒布大学医院中心（UHC）收集的分离株进行全基因组测序（WGS），研究了医院内菌株的分布情况。基于对艰难梭菌谷氨酸脱氢酶（GDH）的粪便抗原筛查试验（RidaQuick CD GDH；德国R - Biopharm AG公司），并经针对A、B和二元毒素的Eazyplex检测（Eazyplex CD检测；德国AmplexDiagnostics GmbH公司）确认，2022年在萨格勒布大学医院中心共纳入了103例首次发生艰难梭菌感染（CDI）的患者。从电子病历中回顾性分析了人口统计学和临床数据。所有样本均进行了WGS分析。根据核心基因组多位点序列分型（core genome MLST），由等位基因差异不超过6个的分离株形成遗传簇。我们鉴定出6个簇，包含2 - 59个分离株、15个单株以及30例可能的医院内传播病例，主要发生在新冠病房。WGS分析被证明有助于识别连接医院不同病房的分离株簇以及医院环境中可能的传播途径。它可用于支持对CDI感染及其传播途径的地方和国家监测。