You Yinwei, Xu Xiao, Liu Hui, Zhang Long
Shandong Key Laboratory for Green Prevention and Control of Agricultural Pests, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Microorganisms. 2024 Dec 4;12(12):2501. doi: 10.3390/microorganisms12122501.
Fungi play an increasingly important role in the biological control of insect pests. XJ1 is highly virulent to locust adults and nymphs, which are a destructive economic pest worldwide. Because of its host association with locusts, which is unique in , in this study, we examined the genetic relationships of XJ1 within . We sequenced the genome of XJ1 and compared it with the genomes of other species. The complete genome of XJ1 is 37.9 Mb, comprising 11,720 putative genes, assembled into eight chromosomes. The genome size is similar to that of other strains. Phylogenomic analysis indicated that XJ1 was most closely related to NRRL3357, not RIB40. Core/pan-genome analysis of XJ1 and other species revealed that XJ1 had 704 strain-specific genes, whereas NRRL3357, KDG 21, and NRRL 2999 had 646, 955, and 779 unique genes, respectively. The XJ1 genome showed structural differences compared with the genomes of RIB40 and NRRL3357 in genomic synteny analysis. These results indicate that XJ1 is genetically distinct at the genome level from other species, including and , and may be as a distinct species. This will provide new insight into the classification of based on genomics.
真菌在害虫的生物防治中发挥着越来越重要的作用。XJ1对蝗虫成虫和若虫具有高毒力，蝗虫是全球一种具有破坏性的经济害虫。由于其与蝗虫的宿主关联在……中是独特的，在本研究中，我们研究了XJ1在……内的遗传关系。我们对XJ1的基因组进行了测序，并将其与其他……物种的基因组进行了比较。XJ1的完整基因组为37.9 Mb，包含11,720个推定基因，组装成8条染色体。基因组大小与其他……菌株相似。系统基因组分析表明，XJ1与NRRL3357关系最为密切，而不是RIB40。对XJ1和其他……物种的核心/泛基因组分析表明，XJ1有704个菌株特异性基因，而NRRL3357、KDG 21和NRRL 2999分别有646、955和779个独特基因。在基因组共线性分析中，XJ1基因组与RIB40和NRRL3357的基因组相比显示出结构差异。这些结果表明，XJ1在基因组水平上与其他……物种（包括……和……）在遗传上不同，可能是一个独特的物种。这将为基于基因组学的……分类提供新的见解。
