新种，一种从制药厂生产环境中分离出的革兰氏阳性、形成芽孢的杆菌，并已添加到MALDI生物分型数据库中。
sp. nov., a Gram-Positive, Spore-Forming Rod Isolated from a Pharmaceutical Facility Production Environment and Added to the MALDI Biotyper Database.
作者信息
Costa Luciana Veloso da, Ramos Juliana Nunes, Albuquerque Leticia de Sousa, Miranda Rebeca Vitória da Silva Lage de, Valadão Talita Bernardo, Veras João Flávio Carneiro, Vieira Erica Miranda Damasio, Forsythe Stephen, Brandão Marcelo Luiz Lima, Vieira Verônica Viana
机构信息
Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, Brazil.
Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, Brazil.
出版信息
Microorganisms. 2024 Dec 5;12(12):2507. doi: 10.3390/microorganisms12122507.
A Gram-positive, aerobic, rod-shaped and spore-forming bacterium strain designation, B190/17, was isolated from an air monitoring sample of a Brazilian immunobiological production facility in 2017. The strain was not identifiable by biochemical methodology VITEK 2 or by MALDI-TOF MS with VITEK MS RUO and MALDI Biotyper. The 16S rRNA gene sequencing results showed 98.51% similarity with FJAT 27215, 98.28% with '' CX 253, 97.96% with MTCC 1458, 97.63% with FJAT 46582 and 97.21% with SGZ8. Biochemical data showed that the strain was alanine arylamidase-, Ala-Phe-Pro arylamidase-, ELLMAN (cysteine residues)-, leucine arylamidase-, phenyalanine arylamidase- and tyrosine arylamidase-positive. The genomic DNA G+C% content of B190/17 was 41.6 mol%. The phylogenetic, genomic taxonomy and biochemical tests suggested that B190/17 represents a novel species and should be classified as the type strain of a novel species. The name sp. nov. was proposed. After characterization, B190/17 was added to the MALDI Biotyper database as sp. nov.
2017年，从巴西一家免疫生物制品生产设施的空气监测样本中分离出一株革兰氏阳性、需氧、杆状且形成芽孢的细菌菌株，命名为B190/17。该菌株无法通过VITEK 2生化方法或使用VITEK MS RUO和MALDI Biotyper的MALDI-TOF MS进行鉴定。16S rRNA基因测序结果显示，该菌株与FJAT 27215的相似度为98.51%，与“CX 253”的相似度为98.28%，与MTCC 1458的相似度为97.96%，与FJAT 46582的相似度为97.63%，与SGZ8的相似度为97.21%。生化数据表明，该菌株的丙氨酸芳基酰胺酶、Ala-Phe-Pro芳基酰胺酶、ELLMAN（半胱氨酸残基）、亮氨酸芳基酰胺酶、苯丙氨酸芳基酰胺酶和酪氨酸芳基酰胺酶呈阳性。B190/17的基因组DNA G+C%含量为41.6 mol%。系统发育、基因组分类学和生化测试表明，B190/17代表一个新物种，应被归类为一个新物种的模式菌株。提出了新物种的名称。经过鉴定后，B190/17作为新物种被添加到MALDI Biotyper数据库中。
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