Qi Hong-Yue, Wang Dan, Han Dongfei, Song Jian, Ali Muhammad, Dai Xiao-Feng, Zhang Xiao-Jun, Chen Jie-Yin
College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang, China.
The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Microbiol. 2023 Jun 2;14:1189354. doi: 10.3389/fmicb.2023.1189354. eCollection 2023.
To establish a safe, efficient, and simple biocontrol measure for gray mold disease caused by , the basic characteristics and antifungal activity of KRS005 were studied from multiple aspects including morphological observation, multilocus sequence analysis and typing (MLSA-MLST), physical-biochemical assays, broad-spectrum inhibitory activities, control efficiency of gray mold, and determination of plant immunity. The strain KRS005, identified as , demonstrated broad-spectrum inhibitory activities against various pathogenic fungi by dual confrontation culture assays, of which the inhibition rate of was up to 90.3%. Notably, through the evaluation of control efficiency, it was found that KRS005 fermentation broth could effectively control the occurrence of tobacco leaves gray mold by determining the lesion diameter and biomass of on tobacco leaves still had a high control effect after dilution of 100 folds. Meanwhile, KRS005 fermentation broth had no impact on the mesophyll tissue of tobacco leaves. Further studies showed that plant defense-related genes involved in reactive oxygen species (ROS), salicylic acid (SA), and jasmonic acid (JA)-related signal pathways were significantly upregulated when tobacco leaves were sprayed with KRS005 cell-free supernatant. In addition, KRS005 could inhibit cell membrane damage and increase the permeability of . Overall, KRS005, as a promising biocontrol agent, would likely serve as an alternative to chemical fungicides to control gray mold.
为建立一种安全、高效且简便的防治由[病原菌名称未给出]引起的灰霉病的生物防治措施，从形态学观察、多位点序列分析与分型（MLSA - MLST）、物理生化测定、广谱抑菌活性、灰霉防治效果以及植物免疫测定等多个方面研究了KRS005的基本特性和抑菌活性。菌株KRS005经鉴定为[具体菌种未给出]，通过对峙培养试验对多种致病真菌表现出广谱抑菌活性，其中对[某种真菌名称未给出]的抑菌率高达90.3%。值得注意的是，通过防治效果评估发现，KRS005发酵液可有效控制烟草叶片灰霉病的发生，通过测定烟草叶片上[病原菌名称未给出]的病斑直径和生物量发现，其在稀释100倍后仍具有较高的防治效果。同时，KRS005发酵液对烟草叶片的叶肉组织没有影响。进一步研究表明，当用KRS005无细胞上清液喷洒烟草叶片时，参与活性氧（ROS）、水杨酸（SA）和茉莉酸（JA）相关信号通路的植物防御相关基因显著上调。此外，KRS005可抑制细胞膜损伤并增加[具体物质未给出]的通透性。总体而言，KRS005作为一种有前景的生物防治剂，有望替代化学杀菌剂用于防治灰霉病。
