Zhang Zhengying, Yang Shu, Li Yanyan, Xie Dian, Chen Guobin, Ren Jiaxu, Zhu Hongmei, Zhou Hantao
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361000, China.
College of Ocean and Earth Sciences, Xiamen University, Xiamen 361000, China.
Microorganisms. 2024 Dec 13;12(12):2574. doi: 10.3390/microorganisms12122574.
The target of rapamycin (TOR) serves as a central regulator of cell growth, coordinating anabolic and catabolic processes in response to nutrient availability, growth factors, and energy supply. Activation of TOR has been shown to promote photosynthesis, growth, and development in yeast, animals, and plants. In this study, the complete cDNA sequence of the gene was obtained from . The structure of LST8 comprises a typical WD40 repeat sequence, exhibiting high sequence similarity to several known LST8 proteins. By overexpressing the gene in , we constructed the NgLst8 transgenic algal strain and measured its photosynthetic activity and growth. We observed that an increase in LST8 abundance promotes the expression of TOR-related kinase, thereby enhancing photosynthetic growth. Transcriptome analysis further elucidated the response mechanism of elevated abundance in relation to photosynthesis. Our findings indicate that increased expression activates ABC transporter proteins and the MAPK signaling pathway, which regulate the transmembrane transport of sugars and other metabolites, integrate photosynthesis, sugar metabolism, and energy signaling, and modulate energy metabolism in algal cells through interactions with the TOR signaling pathway.
雷帕霉素靶蛋白（TOR）作为细胞生长的核心调节因子，根据营养物质的可利用性、生长因子和能量供应来协调合成代谢和分解代谢过程。已证明TOR的激活可促进酵母、动物和植物中的光合作用、生长和发育。在本研究中，该基因的完整cDNA序列是从……获得的。LST8的结构包含典型的WD40重复序列，与几种已知的LST8蛋白具有高度的序列相似性。通过在……中过表达该基因，我们构建了NgLst8转基因藻株，并测量了其光合活性和生长情况。我们观察到LST8丰度的增加促进了TOR相关激酶的表达，从而增强了光合生长。转录组分析进一步阐明了LST8丰度升高与光合作用相关的响应机制。我们的研究结果表明，LST8表达的增加激活了ABC转运蛋白和MAPK信号通路，这些通路调节糖类和其他代谢物的跨膜运输，整合光合作用、糖代谢和能量信号，并通过与TOR信号通路的相互作用调节藻类细胞中的能量代谢。
