Enderlin Dominik, Bieri Uwe, Gadient Jana, Morsy Yasser, Scharl Michael, Rüschoff Jan Hendrik, Hefermehl Lukas John, Nikitin Anna, Langenauer Janine, Engeler Daniel Stephan, Förster Beat, Obrecht Fabian, Surber Jonathan, Scherer Thomas Paul, Eberli Daniel, Poyet Cédric
Department of Urology, University Hospital of Zurich, University of Zurich, 8091 Zurich, Switzerland.
Division of Urology, Department of Surgery, Kantonsspital Baden, 5404 Baden, Switzerland.
Microorganisms. 2024 Dec 15;12(12):2594. doi: 10.3390/microorganisms12122594.
Studies have shown that the human microbiome influences the response to systemic immunotherapy. However, only scarce data exist on the impact of the urinary microbiome on the response rates of bladder cancer (BC) to local instillation therapy. We launched the prospective SILENT-EMPIRE study in 2022 to address this question. We report the results of the pilot study of SILENT-EMPIRE, which aimed to compare the microbiome between fresh frozen (FF) and formalin-fixed paraffin-embedded (FFPE) samples in the cancerous tissue and adjacent healthy tissue of BC patients. Our results show that alpha diversity is increased in FF samples compared to FFPE (coverage index = 0.041, core abundance index = 0.008). No significant differences concerning alpha diversity could be detected between cancerous and non-cancerous tissue in the same BC patients. This study demonstrates that microbiome analysis from both FF and FFPE samples is feasible. Implementing this finding could aid in the translation of research findings into clinical practice.
研究表明，人类微生物组会影响全身免疫治疗的反应。然而，关于泌尿微生物组对膀胱癌（BC）局部灌注治疗反应率影响的数据却极为稀少。我们于2022年启动了前瞻性SILENT-EMPIRE研究以解决这一问题。我们报告了SILENT-EMPIRE试点研究的结果，该研究旨在比较BC患者癌组织和相邻健康组织中新鲜冷冻（FF）样本与福尔马林固定石蜡包埋（FFPE）样本之间的微生物组。我们的结果表明，与FFPE样本相比，FF样本中的α多样性增加（覆盖指数 = 0.041，核心丰度指数 = 0.008）。在同一BC患者的癌组织和非癌组织之间，未检测到关于α多样性的显著差异。这项研究表明，对FF和FFPE样本进行微生物组分析是可行的。落实这一发现有助于将研究结果转化为临床实践。
