Modise-Tlotleng Boitumelo M, Mpoloka Sununguko W, Settypalli Tirumala B K, Hyera Joseph, Kgotlele Tebogo, Kumile Kago, Sechele Mosarwa E, Raboloko Obuile O, Marobela-Raborokgwe Chandapiwa, Viljoen Gerrit J, Cattoli Giovanni, Lamien Charles E
National Agricultural Research and Development Institute, Private Bag 0035, Gaborone, Botswana.
Department of Biological Sciences, University of Botswana, Private Bag 00704, Gaborone, Botswana.
Microorganisms. 2024 Dec 20;12(12):2644. doi: 10.3390/microorganisms12122644.
Abortion is one of the major causes of economic losses in livestock production worldwide. Because several factors can lead to abortion in cattle, sheep and goats, laboratory diagnosis, including the molecular detection of pathogens causing abortion, is often necessary. Bacterial zoonotic diseases such as brucellosis, coxiellosis, leptospirosis, and listeriosis have been implicated in livestock abortion, but they are under diagnosed and under-reported in most developing countries, including Botswana. This study applied a recently developed multiplex high-resolution melting analysis technique, coupled with singleplex qPCR assays, to investigate abortions in livestock in Botswana, using 152 samples from cattle, sheep, and goat abortion cases. spp. were the most frequent pathogen detected, with an overall frequency of 21.1%, followed by with 19.1%. and spp. were not detected in any of specimens samples investigated. Mixed infections with spp. and were observed in 35% specimes examined. There was a good agreement between the multiplex qPCR-HRM and singleplex qPCR for detecting spp. and . This study is the first report on the syndromic testing of abortion-causing pathogens in Botswana. It shows the importance of molecular methods in the differential diagnosis of abortion-causing diseases in domestic ruminants.
流产是全球畜牧业经济损失的主要原因之一。由于多种因素可导致牛、羊和山羊流产，因此通常需要进行实验室诊断，包括对引起流产的病原体进行分子检测。布鲁氏菌病、柯克斯体病、钩端螺旋体病和李斯特菌病等细菌性人畜共患病与家畜流产有关，但在包括博茨瓦纳在内的大多数发展中国家，这些疾病的诊断不足且报告不足。本研究应用最近开发的多重高分辨率熔解分析技术，结合单重定量聚合酶链反应（qPCR）检测方法，对博茨瓦纳152份牛、羊和山羊流产病例样本进行检测，以调查家畜流产情况。检测到的最常见病原体是[具体病原体1]属，总体检出率为21.1%，其次是[具体病原体2]，检出率为19.1%。在所调查的任何样本中均未检测到[具体病原体3]属和[具体病原体4]属。在35%的检测样本中观察到[具体病原体1]属与[具体病原体2]的混合感染。多重qPCR-高分辨率熔解分析（HRM）与单重qPCR在检测[具体病原体1]属和[具体病原体2]方面具有良好的一致性。本研究是博茨瓦纳关于引起流产病原体综合征检测的首次报告。它显示了分子方法在反刍家畜流产病因鉴别诊断中的重要性。
