Peng Peng, Han Fangxin, Gong Xue, Guo Xiangyuan, Su Ying, Zhang Yiwen, Zhan Jingjing
School of Chemical Engineering, Ocean and Life Sciences, Dalian University of Technology, NO.2 Dagong Road, Panjin 124221, China.
School of General Education, Dalian University of Technology, NO.2 Dagong Road, Panjin 124221, China.
Microorganisms. 2024 Dec 22;12(12):2665. doi: 10.3390/microorganisms12122665.
The increasing prevalence of harmful algal blooms (HABs) driven by eutrophication, particularly in China's nearshore waters, is a growing concern. Dinoflagellate blooms have caused significant ecological and economic damage, as well as mass mortality, in cultivated species. Nutrients are one of the primary inducers of blooms. However, the transcriptomic studies of remain sparse, and its metabolic pathways are unknown. This study analyzed the transcriptome of under varying nutrient conditions (nitrogen at 128, 512, and 880 μM; phosphate at 8, 6, and 32 μM), focusing on differential gene expression. The results indicated that deviations in nutrient conditions (higher or lower N:P ratios) led to a higher number of differentially expressed genes compared to the control (N:P ratios = 27.5), thereby underscoring their pivotal role in growth. Gene Ontology (GO) enrichment analyses showed that nutrient limitation upregulated the biosynthesis and catabolism processes while downregulating the cell cycle and division functions. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that, under nitrogen limitation, the proteasome pathways were upregulated, while photosynthesis and carbon fixation were downregulated; under phosphorus limitation, the proteasome pathways were upregulated and nitrogen metabolism was downregulated. These findings suggest that adapts to nutrient stress by adjusting its metabolic processes.
由富营养化驱动的有害藻华（HABs）日益普遍，尤其是在中国近岸水域，这一问题愈发令人担忧。甲藻大量繁殖已对养殖物种造成了重大的生态和经济破坏以及大量死亡。营养物质是藻华的主要诱因之一。然而，关于[具体甲藻名称未给出]的转录组学研究仍然稀少，其代谢途径也尚不明确。本研究分析了[具体甲藻名称未给出]在不同营养条件下（氮浓度分别为128、512和880 μM；磷浓度分别为8、6和32 μM）的转录组，重点关注差异基因表达。结果表明，与对照（氮磷比 = 27.5）相比，营养条件的偏差（较高或较低的氮磷比）导致差异表达基因的数量更多，从而突出了它们在生长中的关键作用。基因本体论（GO）富集分析表明，营养限制上调了生物合成和分解代谢过程，同时下调了细胞周期和分裂功能。京都基因与基因组百科全书（KEGG）分析显示，在氮限制下，蛋白酶体途径上调，而光合作用和碳固定下调；在磷限制下，蛋白酶体途径上调，氮代谢下调。这些发现表明[具体甲藻名称未给出]通过调整其代谢过程来适应营养胁迫。
